Summary
Glucose tolerance and insulin secretion were studied in non-diabetic littermates (n=154) of BB diabetic rats, aged 4–6 months. Initial screening involved two intraperitoneal glucose tolerance tests (0.2g/100g body weight) performed one week apart. Nineteen rats (12%) were found to have impaired tolerance which persisted in 14 (74%) (group 1) and was transient in five animals (group 2). Seven rats progressed to overt diabetes in group 1, but none in group 2. Group 1 was characterized by (a) sustained abnormalities in glucose response to oral and intraperitoneal glucose, as well as intraperitoneal tolbutamide and arginine; (b) fasting hypoinsulinaemia; (c) decreased insulin response to glucose and tolbutamide; (d) suppression of the early and late phases of immunoreactive insulin response to intravenous glucose; (e) no systematic abnormalities in glucagon secretion; and (f) the presence of significant insulitis. The group 2 rats had (a) normal glycaemic response to oral and intraperitoneal glucose, tolbutamide and arginine on further testing; (b) normal fasting insulin but excessive insulin response to glucose and tolbutamide, but not to arginine, and (c) mainly normal islet morphology. Thus, impaired glucose tolerance may occur in BB rats with either hypoinsulinaemia or hyperinsulinaemia.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Nakhooda AF, Like AA, Chappel CI, Murray FT, Marliss EB (1977) The spontaneously diabetic Wistar rat. Metabolic and morphologic studies. Diabetes 26:100–112
Nakhooda AF, Like AA, Chappel CI, Wei C-N, Marliss EB (1978) The spontaneously diabetic Wistar rat (the ‘BB’ rat): Studies prior to and during development of the overt syndrome. Diabetologia 14:199–207
Nakhooda AF, Wei C-N, Like AA, Marliss EB (1978) The spontaneously diabetic Wistar rat (the ‘BB’ rat). A study of rats with transient glycosuria. Diabete Metab 4: 255–259
Marliss EB, Sima AAF, Nakhooda AF (1981) Spontaneous diabetes in the ‘BB’ Wistar rat. In: Martin JM, Ehrlich R, Holland FJ (eds) Etiology and pathogenesis of insulin dependent diabetes mellitus. Raven Press, New York, pp 251–274
Marliss EB, Nakhooda AF, Poussier P, Sima AAF (1982) The diabetic syndrome of the ‘BB’ Wistar rat: Possible relevance to Type 1 (insulin-dependent) diabetes in man. Diabetologia 22: 225–232
Marre M, Bobbioni E, Suarez M, Reach G, Dubois MP, Assan R (1979) Control of gastric glucagon secretion in the acutely pancreatectomized rat. Diabetes 28: 213–220
Nakhooda AF, Wei C-N, Marliss EB (1980) Muscle protein catabolism in diabetes: 3-methylhistidine excretion in the spontaneously diabetic ‘BB’ rat. Metab Clin Exp 29:1272–1277
Nakhooda AF, Sole MJ, Marliss EB (1981) Adrenergic regulation of glucagon and insulin secretion during immobilization stress in normal and spontaneously diabetic BB rats. Am J Physiol 240 (Endocrin Metab 3) E373–378
Poussier P, Nakhooda AF, Sima AAF, Marliss EB (1981) Lymphopenia in the spontaneously diabetic ‘BB’ Wistar rat. Diabetologia 21:317 (Abstract)
Poussier P, Nakhooda AF, Falk JA, Marliss EB (1982) Lymphopenia and abnormal lymphocyte subsets in the ‘BB’ rat: Relationship to the diabetic syndrome. Endocrinology 110:1825–1827
Jackson R, Rassi N, Crump T, Haynes B, Eisenbarth GS (1981) The diabetic rat: profound T-cell lymphocytopenia. Diabetes 30: 887–889
Kobayashi M, Olefsky JM (1979) Effects of streptozotocin induced diabetes on insulin binding, glucose transport and intracellular glucose metabolism in isolated rat adipocytes. Diabetes 28: 87–95
Norfleet NT, Pagliara AS, Haymond MW, Matschinsky F (1975) Comparison of alpha and beta-cell secretory responses in islets isolated with collagenase and in the isolated perfused pancreas of rats. Diabetes 24: 961–970
Pfeifer MA, Halter JB, Porte D Jr (1981) Insulin secretion in diabetes mellitus. Am J Med 70: 579–588
Weir GC, Clore ET, Zmachinski CJ, Bonner-Weir S (1981) Islet secretion in a new experimental model for non-insulin-dependent diabetes. Diabetes 30: 590–595
Kahn CB, Soeldner JS, Gleason RE, Rojas L, Camerini-Davalos RA, Marble A (1963) Clinical and chemical diabetes in offspring of diabetic couples. N Engl J Med 281: 343–347
Tattersall RB, Fajans SS (1975) Prevalence of diabetes and glucose tolerance in 199 offspring of thirty-seven conjugal diabetic parents. Diabetes 24: 452–455
Hollander P, Asplin C, Hansen J, Palmer JP (1980) Beta cell dysfunction in HLA identical siblings of insulin-dependent diabetics. Diabetes 29 (Suppl 2): 54 A (Abstract)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nakhooda, A.F., Poussier, P. & Marliss, E.B. Insulin and glucagon secretion in BB Wistar rats with impaired glucose tolerance. Diabetologia 24, 58–62 (1983). https://doi.org/10.1007/BF00275949
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00275949