Summary
The copolymer of glycidylmethacrylate with ethylene-dimethacrylate, its derivatives, bead cellulose and Sepharose were used for immobilization of DNA via new coupling methods: reaction with oxirane, acylhydrazide hydrazide, and diazonium salt. The susceptibility of immobilized DNA for DNAse was tested. The most efficient immobilization was achieved on carriers with acylhydrazide groups. The binding capacity of the carrier and the susceptibility of immobilized DNA for the DNase depends on the length of the spacer. The immobilized DNA is stable in solution for several weeks, and in the lyophilized state for several months.
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Saudek, V., Drobník, J. & Kálal, J. Immobilization of DNA on poly(glycidyl methacrylate-co-ethylene dimethacrylate), bead cellulose and sepharose. Polymer Bulletin 2, 7–14 (1980). https://doi.org/10.1007/BF00275546
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DOI: https://doi.org/10.1007/BF00275546