Summary
By homogenizing rice leaves in liquid nitrogen, it was possible to isolate intact chloroplasts and, subsequently, pure rice chloroplast DNA from the purified chloroplasts. The DNA was digested by several restriction enzymes and fragments were fractionated by agarose gel electrophoresis. The sum of the fragment sizes generated by the restriction enzymes showed that the total length of the DNA is 130 kb. A circular physical map of fragments, generated by digestion with SalI, PstI, and PvuII, has been constructed. The circular DNA contains two inverted repeats of about 20 kb separated by a large, single copy region of about 75 kb and a short, single copy region of about 15 kb. The location of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase (Fraction I protein) and the 32 KD photosystem II reaction center gene were determined by using as probes tobacco chloroplast DNAs containing these genes. Rice chloroplast DNA differs from chloroplast DNAs of wheat and corn as well as from dicot chloroplast DNAs by having the 32 KD gene located 20 kb removed from the end of an inverted repeat instead of close to the end, as in other plants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bedbrook JR, Bogorad L (1976) Endonuclease recognition sites mapped on Zea mays chloroplast DNA. Proc Natl Acad Sci USA 73:4309–4313
Bowman CM, Koller B, Delius H, Dyer TA (1981) A physical map of wheat chloroplast DNA showing the location of the structural genes for the ribosomal RNAs and the large subunit of ribulose 1,5-bisphosphate carboxylase. Mol Gen Genet 183:93–101
Fluhr R, Edelman M (1981) Physical mapping of Nicotiana tabacum chloroplast DNA. Mol Gen Genet 181:484–490
Herrmann RG, Whitfeld PR, Bottomley W (1980) Construction of a Sal/PstI restriction map of spinach chloroplast DNA using low-gelling-temperature-agarose electrophoresis. Gene 8:179–191
Larrinua IM, Muskavitch KMT, Gubbins EJ, Bogorad L (1983) A detailed restriction endonuclease site map of the Zea mays plastid genome. Plant Mol Biol 2:129–140
Palmer JD, Thompson WF (1982) Chloroplast DNA rearrangements are more frequent when a large inverted repeat sequence is lost. Cell 29:537–550
Saltz Y, Beckman J (1981) Chloroplast DNA preparation from Petunia and Nicotiana. Plant Mol Biol Newslett 2:73–74
Shinozaki K, Sugiura M (1982) The nucleotide sequence of the tobacco chloroplast gene for the large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase. Gene 20:91–102
Spielmann A, Ortiz W, Stutz E (1983) The soybean chloroplast genome: construction of a circular restriction site map and location of DNA regions encoding the genes for rRNAs, the large subunit of the ribulose-1,5-bisphosphate carboxylase and the 32 KD protein of the photosystem II reaction center. Mol Gen Genet 190:5–12
Sugiura M, Kusuda J (1979) Molecular cloning of tobacco chloroplast ribosomal RNA genes. Mol Gen Genet 172:137–141
Vieira J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
Author information
Authors and Affiliations
Additional information
Communicated by K. Tsunewaki
Rights and permissions
About this article
Cite this article
Hirai, A., Ishibashi, T., Morikami, A. et al. Rice chloroplast DNA: a physical map and the location of the genes for the large subunit of ribulose 1,5-bisphosphate carboxylase and the 32 KD photosystem II reaction center protein. Theoret. Appl. Genetics 70, 117–122 (1985). https://doi.org/10.1007/BF00275309
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00275309