Summary
A method is described for transfection (genetic transformation) of barley caryopsis electrophoretically with DNA. β-Glucuronidase activity was detected after the electrophoretic transfection with plasmid pBI221 DNA carrying the cauliflower mosaic virus promotor and bacterial β-glucuronidase coding sequence. Electrophoretic transfection is evidently effective with pieces of callus and seeds of many plants.
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Communicated by P.M.A. Tigerstedt
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Ahokas, H. Transfection of germinating barley seed electrophoretically with exogenous DNA. Theoret. Appl. Genetics 77, 469–472 (1989). https://doi.org/10.1007/BF00274265
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DOI: https://doi.org/10.1007/BF00274265