Summary
Autologous transfusions of 111indium-labelled peripheral blood lymphocytes reportedly image the pancreas of patients with Type 1 (insulin-dependent) diabetes at the time of onset. We attempted to apply this technique to the spontaneously diabetic BB/W rat. First, acutely diabetic BB/W rats, diabetes-prone BB/W rats, diabetes-resistant W-line BB/ W rats, and Wistar Furth rats were given autologous transfusions of labelled peripheral blood lymphocytes. Radioactivity recovered from the pancreas was similar in all groups. No correlation was found between the intensity of imaging and the presence or intensity of insulitis. To decrease non-specific intravascular radioactivity, acutely diabetic, diabetes-prone, and W-line rats were perfused 48 h after autologous transfusion of labelled lymphocytes. Again, the intensity of recovered activity was similar in all groups, using both macroautoradiography and numerical counting techniques. A second set of experiments studied diabetes and insulitis induced by passive transfer of concanavalin A-treated splenic lymphocytes from acutely diabetic donors. Activated lymphocytes were labelled with 111Indium and given to groups of diabetes-prone and diabetes-resistant rats. There were no differences in pancreatic localization 72–96 h after injection. Groups of diabetes-prone and diabetes-resistant rats were also given concanavalin A-activated lymphocytes and then challenged 2–10 days later with autologous transfusions of labelled peripheral blood lymphocytes. Again, no differences in organ labelling or imaging were detected. We conclude that the autologous transfusions of 111indium-labelled lymphocytes do not label or image the pancreas of the BB/W rat.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Cahill GF, McDevitt HO (1981) Insulin-dependent diabetes mellitus: the initial lesion. N Engl J Med 304: 1454–1465
Gepts W (1980) Islet cell morphology in type I and type II diabetes. In: Irvine WJ (ed) Immunology of diabetes. Teviot, Scientific Publications, Edinburgh, pp 255–366
Goodwin DA (1978) Cell labeling with oxine chelates of radioactive metal ions: techniques and clinical implications. J Nucl Med 19: 557–559
Lavender JP, Goldman JM, Arnot RN, Thakur ML (1977) Kinetics of indium-111-labelled lymphocytes in normal subjects and patients with Hodgkin's disease. Br Med J 2: 797–799
Riba AL, Thakur ML, Gottschalk A, Andriole VT, Zaret BL (1979) Imaging experimental infective endocarditis with 111indium-labeled blood cellular components. Circulation 59: 336–343
Bergmann SR, Lerch RA, Carlson EM, Saffitz JE, Sobel BE (1982) Detection of cardiac transplant rejection with radiolabelled lymphocytes. Circulation 65: 591–599
Kaldany A, Hill T, Wentworth S, Brink SJ, D'Elia JA, Clouse M, Soeldner JS (1982) Trapping of peripheral blood lymphocytes in the pancreas of patients with acute-onset insulin-dependent diabetes mellitus. Diabetes 31: 463–466
Nakhooda AF, Like AA, Chappel CI, Wei CN, Marliss EB (1978) The spontaneously diabetic Wistar rat (the “BB” rat): studies prior to and during development of the overt syndrome. Diabetologia 14: 199–207
Nakhooda AF, Like AA, Chappel CI, Murray FT, Marliss EB (1977) The spontaneously diabetic Wistar rat. Metabolic and morphologic studies. Diabetes 26: 100–112
Koevary S, Rossini AA, Stoller W, Chick W, Williams RM (1983) Passive transfer of diabetes in the BB/W rat. Science 220: 727–728
Wagstaff J, Gibson C, Thatcher N, Ford WL, Sharma H, Benson W, Crowther D (1981) A method for following human lymphocyte traffic using 111indium oxine labelling. Clin Exp Immunol 43: 435–442
Spence JT, Underwood BJ, Duncan CP, Cotton JW (1968) Correlation method versus experimental method. In: Elliott RM, Lindzey G, MacCorquodale K (eds) Elementary statistics. Appleton-Century-Crofts, New York, pp 113–132
Frost P, Smith J, Frosh H (1978) The radiolabelling of lymphocytes and tumor cells with 111Indium. Proc Soc Exp Biol Med 157: 61–65
Altaian PK, Dittmer DS (1961) Blood volumes. In: Altaian PL, Dittmer DS (eds) Blood and other body fluids. Federation of the American Society for Experimental Biology, Washington, p 5
Sparshott SM, Sharma H, Kelly JD, Ford WL (1981) Factors influencing the fate of 111indium-labelled lymphocytes after transfer to syngeneic rats. J Immunol Methods 41: 303–320
Sternthal E, Like AA, Sarantis K, Braverman LE (1981) Lymphocytic thyroiditis and diabetes in the BB/W rat. Diabetes 30: 1058–1061
Like AA, Weringer EJ, Holdash A, McGill P, Rossini AA (1983) Nature of resistance to autoimmune diabetes in Biobreeding/ Worcester control rats. Diabetologia 25: 175 (Abstract)
Thakur ML, Coleman RE, Welch MJ (1977) 111Indium-labelled leucocytes for the localization of abscesses: preparation, analysis, tissue distribution and comparison with 67gallium-citrate in dogs. J Lab Clin Med 89: 217–228
Segal AW, Deteix P, Garcia R, Tooth P, Zanelli GD, Allison AC (1979) 111Indium labelling of lymphocytes: a detrimental effect on neutrophil and lymphocyte function and an improved method of cell labelling. J Nucl Med 19: 1238–1244
Berge RJM ten, Natarajan AT, Harderman MR, Royen EA van, Schellekens PThA (1983) Labelling with 111indium has detrimental effects on human lymphocytes: concise communication. J Nucl Med 24: 615–620
Mordes JP, Nakano K, Gallina DL, Like AA, Rossini AA (1984) Irradiation of activated lymphocytes from BB rats prevents adoptive transfer of diabetes but does not vaccinate against spontaneous diabetes. Clin Res 32: 404A (Abstract)
Pozzilli P, Pantano P, Negri M, Andreani D, Cudworth AG (1983) Tracking of 111indium-oxine labelled lymphocytes in autoimmune thyroid disease. Clin Endocrinol 19: 111–117
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gallina, D.L., Pelletier, D., Doherty, P. et al. 111Indium-labelled lymphocytes do not image or label the pancreas of the BB/W rat. Diabetologia 28, 143–147 (1985). https://doi.org/10.1007/BF00273861
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00273861