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Restriction of streptomyces phage SH5 by endonuclease ShyI from Streptomyces hygroscopicus 0477

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Summary

DNA of the temperate Streptomyces phage SH5 (DNA molecular weight 27x106) is subject to restriction-modification mediated by S. hygroscopicus 0477, S. levoris 1331 and 2340. The restriction endonuclease ShyI (isoschizomeric with SacII) isolated from S. hygroscopicus 0477 is involved in restriction of SH5·1331 and SH5·2340 DNAs in S. hygroscopicus 0477.

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References

  • Adams MH (1959) Bacteriophages. Wiley Interscience, New York

    Google Scholar 

  • Bradley DE (1967) Ultrastructure of bacteriophages and bacteriocins. Bacteriol Rev 31:230–314

    Google Scholar 

  • Chater KF, Carter AT (1978) Restriction of a bacteriophage in Streptomyces albus P (CMI 52766) by endonuclease SalPI. J Gen Microbiol 109:181–185

    Google Scholar 

  • Chater KF, Wilde LC (1976) Restriction of a bacteriophage of Streptomyces albus G involving endonuclease SalI. J Bacteriol 128:644–650

    Google Scholar 

  • Chater KF, Wilde LC (1980) Streptomyces albus G mutants defective in the SalGI restriction-modification system. J Gen Microbiol 116:323–334

    Google Scholar 

  • Greene PJ, Heyneker HL, Bolivar F, Rodriguez RL, Betlach MC, Covarrubias AA, Backman K, Russel DJ, Tait R, Boyer HW (1978) A general method for the purification of restriction enzymes. Nucl Acid Res 5:2373–2380

    Google Scholar 

  • Helling RB, Goodman HM, Boyer HW (1974) Analysis of endonuclease EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose gel electrophoresis. J Virol 14:1235–1244

    Google Scholar 

  • Kan NC, Lautenberger JA, Edgell MH, Hutchison CA (1979) III. The nucleotide sequence recognized by the Escherichia coli K12 restriction and modification enzymes. J Mol Biol 130:191–209

    Google Scholar 

  • Klaus S, Süss F, Juch C, Hauck A (1978) Characterization of virulent actinophage S2. Z Allg Mikrobiol 18:575–586

    Google Scholar 

  • Klaus S, Triebel H, Hartmann M, Walter F, Zöpel P, Bär H (1979) Molecular characterization of the genomes of actinophages SH3, SH10, SH11, and SH12 infecting Streptomyces hygroscopicus. Mol Gen Genet 172:319–327

    Google Scholar 

  • Krüger DH, Hansen S, Schroeder C (1980) Virusadaptation an Wirtszellen: Die nicht-klassische Modifizierung des T3 Phagen. Z Allg Mikrobiol 8:495–502

    Google Scholar 

  • Lomovskaya ND, Chater KF, Mkrtumian NM (1980) Genetics and molecular biology of Streptomyces bacteriophages. Microbiological Rev 44:206–229

    Google Scholar 

  • Rautenstein YI, Solovyeva NY, Roth M, Noack D, Klaus S (1976) Lysogeny of a strain of Streptomyces hygroscopicus producing turimycin and some features of its temperate phage. Mikrobiologiya 45:922–925

    Google Scholar 

  • Roberts RJ (1980) Restriction and modification enzymes and their recognition sequences. Gene 8:329–343

    Google Scholar 

  • Roth M, Noack D, Solovycva NY, Rautenstein YI (1978) Effective enrichment of the temperate actinophage SH3 and its interaction with Streptomyces hygroscopicus. Z Allg Mikrobiol 18:147–149

    Google Scholar 

  • Solovyeva NY, Rautenstein YI (1978) On the phenomenon of temperate phages for the lysogenic Streptomyces hygroscopicus culture. Mikrobiologiya 47:956–958

    Google Scholar 

  • Solovyeva NY, Rautenstein YI (1980) On the restriction and modification of actinophages by the culture of Streptomyces hygroscopicus and Streptomyces levoris. Mikrobiologiya 49:512–515

    Google Scholar 

  • Voeykova TA, Slavinskaya EV, Orekhov AV, Lomovskaya ND (1979) Identification of restriction and modification systems in Streptomyces strains. Genetika 15:1746–1756

    Google Scholar 

  • Walter F, Hartmann M, Roth M (1978) Purification and characterization of a site specific endonuclease from Streptomyces hygroscopicus. 12th FEBS Meeting, Dresden, Abstracts 0648

  • Walter F, Hartmann M, Klaus S (1981) Restriction endonuclease analysis of DNA from the Streptomyces phages SH3, SH5, SH10, and SH13. Gene 13:57–63

    Google Scholar 

  • Westmoreland BC, Szybalski W, Ris H (1969) Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage Lambda by electron microscopy. Science 163:1343–1346

    Google Scholar 

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Authors and Affiliations

  1. Forschungszentrum für Molekularbiologie und Medizin, Zentralinstitut für Mikrobiologie und experimentelle Therapie, Akademie der Wissenschaften der DDR, Beutenbergstraße 11, DDR-69, Jena, German Democratic Republic

    Siegfried Klaus, Manfred Hartmann, Hans Krügel, Martin Roth & Friedrich Walter

  2. Institut Mikrobiologii AN USSR, Moscow

    Yacov I. Rautenstein & Nina Y. Solovyeva

Authors
  1. Siegfried Klaus
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  2. Manfred Hartmann
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  3. Hans Krügel
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  4. Martin Roth
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  5. Friedrich Walter
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  6. Yacov I. Rautenstein
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  7. Nina Y. Solovyeva
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Communicated by H. Böhme

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Klaus, S., Hartmann, M., Krügel, H. et al. Restriction of streptomyces phage SH5 by endonuclease ShyI from Streptomyces hygroscopicus 0477. Molec. Gen. Genet. 184, 286–288 (1981). https://doi.org/10.1007/BF00272919

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  • DOI: https://doi.org/10.1007/BF00272919

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