Summary
Two plasmids were constructed consisting of the E. coli vector pACYC184 and the cyanobacterial plasmid pUC1. These recombinants, designated pUC104 and pUC105, can be transformed to E. coli K12 as well as to the cyanobacterium Anacystis nidulans R2 and in both hosts they express their antibiotic markers. pUC104 and pUC105 differ with respect to the location and the orientation of the pACYC184 segment in pUC1. pUC104 was found to be stable under all circumstances. Transformation of pUC105 to A. nidulans R2 gave intact plasmids when chloramphenicol was the selective agent, but upon ampicillin selection a deletion derivative was produced identical to pUC1. Further characteristics of pUC104 and pUC105 are described and their usefulness as cloning vectors is discussed.
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References
Alton NK, Vapnek D (1979) Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9. Nature 282:864–869
Astier C, Espardellier F (1976) Mise en évidence d'un système de transfert génétique chez une cyanophycée du genre Aphanocapsa. CR Acad Sci Paris 282:795–797
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Boyer HW, Rouilland-Dussoix D (1969) A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41:459–472
Chakrabarty AM (1976) Plasmids in Pseudomonas. Ann Rev Genet 10:7–30
Chang ACY, Cohen SN (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic plasmid. J Bacteriol 134:1141–1156
Cohen SN, Chang ACY, Hsu L (1972) Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci USA 69:2110–2114
Collins J, Hohn B (1978) Cosmids: A type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage λ heads. Proc Natl Acad Sci USA 75:4242–4246
Devilly CI, Houghton JA (1977) A study of genetic transformation in Gloeocapsa alpicola. J Gen Microbiol 98:277–280
Ehrlich SD (1977) Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis. Proc Natl Acad Sci USA 74:1680–1682
Geier GE, Modrich P (1979) Recognition sequence of the dam methylase of Escherichia coli K 12 and mode of cleavage of DpnI endonuclease. J Biol Chem 254:1408–1413
Guerry P, LeBlanc DJ, Falkow S (1973) General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol 116:1064–1066
Lau RH, Doolittle WF (1979) Covalently closed circular DNAs in closely related unicellular cyanobacteria. J Bacteriol 137:648–652
Lau RH, Doolittle WF (1980) Cyanobacterial plasmids: their widespread occurrence, and the existence of regions of homology between plasmids in the same and different species. Mol Gen Genet 178:203–211
Murray NE, Bruce SA, Murray K (1979) Molecular cloning of the DNA ligase gene from bacteriophage T4. II. Amplification and preparation of the gene product. J Mol Biol 132:493–505
Reaston J, Van den Hondel CAMJJ, Van der Ende A, Van Arkel GA, Stewart WDP, Herdman M (1980) Comparison of plasmids from the cyanobacterium Nostoc PCC7524 with two mutant strains unable to form heterocysts. FEMS Microbiol Lett 9:185–188
Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61
Roberts RJ (1981) Restriction and modification enzymes and their recognition sites. Nucleic Acids Res 9:r75-r96
Roberts TM, Koth KE (1976) The blue green alga Agmenellum quadruplicatum contains covalently closed DNA circles. Cell 9:551–557
Shestakov SV, Khyen NG (1970) Evidence for genetic transformation in blue green alga Anacystic nidulans. Mol Gen Genet 107:372–375
Simon RD (1978) Survey of extrachromosomal DNA found in the filamentous cyanobacteria. J Bacteriol 136:414–418
Stevens SE, Porter RD (1980) Transformation in Agmenellum quadruplicatum. Proc Natl Acad Sci USA 77:6052–6056
Stüber D, Bujard H (1981) Organization of transcriptional signals in plasmids pBR322 and pACYC184. Proc Natl Acad Sci USA 78:167–171
Timmis KN, Cohen SN, Cabello FC (1978) DNA cloning and the analysis of plasmid structure and function. Prog Mol Subcell Biol 6:1–58
Van den Hondel CAMJJ, Van Arkel GA (1980) Development of a cloning system in cyanobacteria. Anthonie van Leeuwenhoek 46:228–229
Van den Hondel CAMJJ, Keegstra W, Borrias WE, Van Arkcl GA (1979) Homology of plasmids in strains of unicellular cyanobacteria. Plasmid 2:323–333
Van den Hondel CAMJJ, Verbeek S, Van der Ende A, Weisbeek PJ, Borrias WE, Van Arkel GA (1980) Introduction of transposon Tn901 into a plasmid of Anacystis nidulans: preparation for cloning in cyanobacteria. Proc Natl Acad Sci USA 77:1570–1574
Van Embden JDA (1978) Translocation of an ampicillin resistance determinant within an R-factor aggregate in Salmonella panama. Anthonie van Leeuwenhoek 44:203–218
Watanabe T (1963) Infective heredity of multiple drug resistance in bacteria. Bacteriol Rev 27:87–115
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Kuhlemeier, C.J., Borrias, W.E., van den Hondel, C.A.M.J.J. et al. Vectors for cloning in cyanobacteria: Construction and characterization of two recombinant plasmids capable of transformation to Escherichia coli K12 and Anacystis nidulans R2. Molec. Gen. Genet. 184, 249–254 (1981). https://doi.org/10.1007/BF00272912
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DOI: https://doi.org/10.1007/BF00272912