Summary
The specialized transducing bacteriophage λdpyrE DNA was used as a source of DNA to clone two ribosomal protein genes rpmB (L28) and rpmG (L33) on the cloning vehicle pACYC184. Using one of these plasmids, the nucleotide sequence of these two genes and their flanking regions were determined. The amino acid sequences of both proteins deduced from the nucleotide sequences match with the amino acid sequences previously determined, with one exception. The nucleotide sequences suggest that these two ribosomal protein genes are cotranstribed. There was no expression of the second gene of the operon, rpmG, in the absence of the 5′ sequences adjacent to the first gene, rmpB. Observation of the structure of mRNA also strongly supports the idea that rpmB and rpmG are in a single transcription unit whose order is: rpmBp-rpmB-rpmG-rpmGt.
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References
Adler HI, Fisher WD, Cohen A, Hardigree AA (1967) Miniature Escherichia coli cells deficient in DNA. Proc Natl Acad Sci USA 57:321–326
An G, Friesen JD (1980) Characterization of promoter-cloning plasmids: Analysis of operon structure in the rif region of Escherichia coli and isolation of an enhanced internal promoter mutant. J Bacteriol 144:904–916
An G, Justesen J, Watson RJ, Friesen JD (1979) Cloning the spoT gene of Escherichia coli; Identification of the spoT gene product. J Bacteriol 137:1100–1110
Anderson EH (1946) Growth requirements of virus resistant E. coli strain B. Proc Natl Acad Sci USA 32:120–122
Bachmann B, Low KB (1980) Linkage map of Escherichia coli K-12. Microbiol Rev 44:1–56
Berk AJ, Sharp PA (1978) Spliced early mRNAs of simian virus 40. Proc Natl Acad Sci USA 75:1274–1278
Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW (1977) Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
Casadaban MJ, Cohen SN (1980) Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol 138:179–207
Casey J, Davidson J (1976) Rates of formation and thermal stabilities of RNA:DNA and DNA:RNA duplexes at high concentrations of formamide. Nucl Acid Res 4:1539–1552
Chang ACY, Cohen SN (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from P15A cryptic mini-plasmids. J Bacteriol 134:1141–1156
Fiil NP, Willumsen BM, Friesen JD, Von Meyenburg K (1977) Interaction of alleles of the relA, relC, and spoT genes in Escherichia coli; Analysis of the interconversion of GTP, ppGpp and pppGpp. Mol Gen Genet 150:87–101
Grantham R, Gautier C, Gouy M (1980) Codon frequencies in 119 individual genes confirm consistent choices of degenerate bases according to genome type. Nucl Acids Res 8:1893–1912
Hardy SJS, Kurland CG, Voynow P, Mora G (1969) The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochem 8:2897–2905
Isono K, Schnier J, Kitakawa M (1980) Genetic fine structure of the pyrE region containing the genes for ribosomal proteins L28 and L33 in Escherichia coli. Mol Gen Genet 179:311–317
Kaltschmidt E, Wittmann HG (1970) Ribosomal proteins. VII. Two dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Ann Biol Chem 36:401–412
Kirschbaum JB, Konrad EB (1973) Isolation of a specialized transducing bacteriophage carrying the beta subunit gene for E. coli RNA polymerase. J Bacteriol 116:517–526
Kitakawa M, Blumenthal L, Isono K (1980) Isolation and characterization of specialized transducing lambda phages carrying ribosomal protein genes of Escherichia coli. Mol Gen Genet 180:343–349
Lennox ES (1955) Transduction of linked genetic characters of the host by bacteriophage Pl. Virology 1:190–206
Mandel M, Higa A (1970) Calcium-dependent bacteriophage DNA infection. J Mol Biol 73:453–471
Maniatis T, Jeffrey A, van de Sande H (1975) Chain length determination of small double and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochem 14:3787–3794
Manley JL, Sharp PA, Gefter ML (1979) RNA synthesis in isolated nuclei: In vitro initiation of adenovirus 2 major late mRNA precursor. Proc Natl Acad Sci USA 76:160–164
Maxam AM, Gilbert W (1980) Sequencing end-labelled DNA with base-specific chemical cleavages. In: Meth Enzymol 65:499–560
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Post LE, Nomura M (1980) DNA sequences the str operon of Escherichia coli. J Biol Chem 255:4660–4666
Post LE, Arfsten AE, Davis GR, Nomura M (1980) DNA sequence of the promoter region for the α ribosomal operon in Escherichia coli. J Biol Chem 255:4653–4659
Post LE, Arfsten AE, Reusser F, Nomura M (1978) DNA sequences of promoter regions for the str and spc ribosomal protein operons in E. coli. Cell 15:215–229
Post LE, Strycharz GD, Nomura M, Lewis H, Dennis PP (1979) Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase beta in Escherichia coli. Proc Natl Acad Sci USA 76:1697–1901
Pribnow D (1975) Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc Natl Acad Sci USA 72:784–788
Rosenberg M, Court D (1979) Regulatory sequence involved in the promotion and termination of RNA transcription. Annu Rev Genet 13:319–353
Sancar A, Hack AM, Rupp D (1979) Single method for identification of plasmid-coded proteins. J Bacteriol 137:692–693
Shinte J, Dalgarno L (1974) The 3′ terminal sequence of Escherichia coli 16S ribosomal RNA: Complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci USA 71:1342–1346
Travers AA (1980) Promoter sequence for stringent control of bacterial ribonucleic acid synthesis. J Bacteriol 141:973–976
Wittmann-Liebold B, Marzinzig E (1977) Primary structure of protein L28 from the large subunit of Escherichia coli ribosomes. FEBS Lett 81:214–217
Wittmann-Liebold B, Pannenbecker R (1976) Primary structure of proteins L33 from the large subunit of the Escherichia coli ribosome. FEBS Lett 68:115–118
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Communicated by A. Böck
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Lee, J.S., An, G., Friesen, J.D. et al. Cloning and the nucleotide sequence of the genes for Escherichia coli ribosomal proteins L28 (rpmB) and L33 (rmpG). Molec. Gen. Genet. 184, 218–223 (1981). https://doi.org/10.1007/BF00272908
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DOI: https://doi.org/10.1007/BF00272908