Summary
Three electrophoretic variants of the peroxidase b isoenzymes in Petunia have been found. The encoding gene prxB is shown to be located on chromosome I by its linkage with the gene Hfl. Analysis of prxB heterozygotes showed a gradual increase of the electrophoretic mobility of all three PRXb allozymes during development and differential expression in enzyme activity of three prxB alleles. The location of prxB on chromosome I was confirmed by an allelic dosage effect in trisomies I, trisomie segregation and the construction of trisomies I with triple-banded PRXb phenotype. From telotrisomic analysis it was concluded that prxB and Hfl are located on the same arm of chromosome I. The unexpected linkage of prxB and Hfl with the gene Fl in one of the crosses was suggested to be caused by a translocation in line SI, involving the gene Fl.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature
Berg, B.M. van den; Wijsman, H.J.W. (1981): Genetics of the peroxidase isoenzymes in Petunia. Part 1: Organ specificity and general genetic aspects of the peroxidase isoenzymes. Theor. Appl. Genet. 60, 71–76
Birchler, J.A. (1979): A study of enzyme activities in a dosage series of the long arm of chromosome one in Maize. Genetics 92, 1211–1229
Fobes, J.F. (1980): Trisomic analysis of isozymic loci in Tomato species: segregation and dosage effects. Biochem. Genet. 18, 401–421
Hart, G.J.; Langsten, P.J. (1977): Chromosomal location and evolution of isozyme structural genes in hexaploid Wheat. Heredity 39, 263–277
Jong, J.H. de; de Bock, T.S.M. (1978): Use of haploids of Beta vulgaris L. for the study of orcein and giemsa stained chromosomes. Euphytica 27, 41–47
Lewis, E.J.; Humphreys, M.W.; Caton, M.P. (1980): Chromosome location of two isozymic loci in Lolium perenne using primary trisomies. Theor. Appl. Genet. 57, 237–239
Maizonnier, D.; Cornu, A. (1971): A telocentric translocation responsible for variegation in Petunia. Genetica 42, 422–436
Maizonnier, D. (1976): Etude cytogénétique de variations chromosomiques naturelles ou induites chez Petunia hybrida Hort. Thèse d'Etat. Univ. Dijon, France
Maizonnier, D.; Moessner, A. (1979): Localization of the linkage groups on the seven chromosomes of the Petunia hybrida genome. Genetica 51, 143–148
McMillin, D.E.; Roupakias, D.G.; Scandalios, J.G. (1979): Chromosomal location of two mitochrondrial malate dehydrogenase structural genes in Zea mays using trisomies and B-A translocations. Genetics 92, 1241–1250
Nielsen, G.; Frydenberg, O. (1971): Chromosome localization of the esterase loci Est-1 and Est-2 in Barley by means of trisomies. Hereditas 67, 152–154
Nielsen, G.; Scandalios, J.G. (1974): Chromosomal location by use of trisomies and new alleles of an endopeptidase in Zea mays. Genetics 77, 679–686
Roupakias, D.G.; McMillin, D.E.; Scandalios, J.G. (1980): Chromosomal location of the catalase structural genes in Zea mays, using B-A translocations. Theor. Appl. Genet. 58, 211–218
Sybenga, J. (1972): General Cytogenetics. Amsterdam: North Holland.
Skan, S.A. (1918): Petunia integrifolia, Curtis's Botanical Magazine 144, Tab. 8749
Wiering, H. (1974): Genetics of flower colour in Petunia hybrida Hort. Genen Phaenen 17, 117–134
Wolf, G.; Rimpau, J.; Lelley, T. (1977): Localization of structural and regulatory genes of phosphodiesterase in Wheat. Genetics 86, 597–605
Author information
Authors and Affiliations
Additional information
Communicated by D. von Wettstein
Rights and permissions
About this article
Cite this article
van den Berg, B.M., Wijsman, H.J.W. Genetics of the peroxidase isoenzymes in Petunia. Theoret. Appl. Genetics 61, 297–303 (1982). https://doi.org/10.1007/BF00272844
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00272844