Summary
It is shown by quantitative electron-microscopical analysis that veincleared leaves from plants systemically infected with TMV contain a majority of cells in the same stage of infection. Under defined environmental conditions the leaves which will show this synchronized virus synthesis can be determined at the time of inoculation. A standardized procedure for routine use of the system is described.
The host-virus system has been used to investigate the sequential development of the virus-induced cytoplasmic differentiations and inclusion bodies. The rapid phase of virus particle formation commences at veinclearing and continues for about 40 hours. The first recognizable cytoplasmic change consists of a local branching and folding of the endoplasmic reticulum. This differentiation develops into the inclusion with diffuse, flexible rods found in late stages of infection together with the virus crystals.
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Nilsson-Tillgren, T., Kolehmainen-Sevéus, L. & von Wettstein, D. Studies on the biosynthesis of TMV. Molec. Gen. Genet. 104, 124–141 (1969). https://doi.org/10.1007/BF00272793
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DOI: https://doi.org/10.1007/BF00272793