Summary
The present mapping studies in the gal region of E. coli are a continuation of experiments reported previously (Pfeifer and Oellermann, 1967).
By mapping of 238 gal mutants against 145 deletions of the gal region carried by λdg phages the kinase gene could be divided into 14 and the transferase gene into 19 deletion groups1. Results of quantitative transduction experiments strongly suggest that there are preferred endpoints for the deletions.
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References
Davison, J., R. Frame, and J. Bishop: A partial deletion map of the galactose operon in E. coli K12. Genet. Res. 10, 107–116 (1967).
Pfeifer, D., and R. Oellermann: Mapping of gal--mutants by transducing λdg carrying deletions of the gal region. Molec. Gen. Genetics 99, 248–252 (1967).
Sussman, R., et F. Jacob: Sur un système de répression thermosensible chez le bacteriophage d'Escherichia coli. C.R. Acad. Sci. (Paris) 254, 1517 (1962).
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Communicated by G. Bertani
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Pfeifer, D., Rosypal, S. Deletion mapping of galactose mutants with transducing λ phages. Molec. Gen. Genet. 104, 116–123 (1969). https://doi.org/10.1007/BF00272792
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DOI: https://doi.org/10.1007/BF00272792