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Cell regeneration and sustained division of protoplasts from cotton (Gossypium hirsutum L.)

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Abstract

Protoplasts were isolated from 12 day old subcultured phytohormone habituated callus tissue of Gossypium hirsutum L. (0.5% cellulysin-Calbiochem, 0.6% macerase-Calbiochem, 0.7M mannitol, and pH 5.0). After separation and purification (0.35M sucrose floatation medium), the protoplasts were cultured (K3 media of Kao et al., 1974 with 0.9 μM BAP, 5 μM IAA and 0.35M sucrose) in both liquid and solid medium at a density of 5×105 protoplasts/ml. Four weeks after isolation, cell regeneration and callus formation was observed.

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Abbreviations

IAA:

indoleacetic acid

BAP:

6-benzyl-adenine

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Author notes

  1. John C. Thomas

    Present address: Department of Biology, Texas A & W University, 77843, College Station, TX, USA

Authors and Affiliations

  1. University of Arizona, Forbes Building No. 36, Room 201, 85721, Tucson, AZ, USA

    Kamel Saka, Frank R. Katterman & John C. Thomas

Authors
  1. Kamel Saka
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  2. Frank R. Katterman
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  3. John C. Thomas
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Additional information

Communicated by J.M. Widholm

Arizona Experimental Station Publication No. 4373

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Saka, K., Katterman, F.R. & Thomas, J.C. Cell regeneration and sustained division of protoplasts from cotton (Gossypium hirsutum L.). Plant Cell Reports 6, 470–472 (1987). https://doi.org/10.1007/BF00272785

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  • DOI: https://doi.org/10.1007/BF00272785

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