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Somatic embryogenesis and plant regeneration from the anther of Aconitum carmichaeli Debx

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Abstract

Anthers of Aconitum carmichaeli Debx. were used for callus induction. After the addition of 5 ppm 2,4-D and 1 ppm kinetin callus induction occurred over a period of 15 weeks. When calluses were subcultured on a medium containing 1 ppm 2,4-D for 12 weeks embryogenesis occurred. Mature somatic embryos developed normal shoots when transferred to basal medium inoculated with 1 ppm GA and 5 ppm BAP. Rooting occurred after the transfer of shoots to a new medium containing 0.5 ppm IAA and plantlets formed. The transplantation of these was successful and all plants matured during 5 months subsequent cultivation.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Kyushu University, Higashi-ku, 812, Fukuoka, Japan

    K. Hatano, Y. Shoyama & I. Nishioka

Authors
  1. K. Hatano
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  2. Y. Shoyama
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  3. I. Nishioka
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Communicated by M. Tabata

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Hatano, K., Shoyama, Y. & Nishioka, I. Somatic embryogenesis and plant regeneration from the anther of Aconitum carmichaeli Debx. Plant Cell Reports 6, 446–448 (1987). https://doi.org/10.1007/BF00272779

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  • DOI: https://doi.org/10.1007/BF00272779

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