Abstract
Immature embryos of Fagopyrum esculentum cv. Pennquad were isolated from field-grown plants and cultured on media containing a high benzylaminopurine to indole-3-acetic acid ratio. Part of the embryos were grown in the presence of 2,4-dichlorophenoxyacetic acid and kinetin for the first 5 days, and then transferred to benzylaminopurine + indole-3-acetic acid medium. From callus tissues developed on hypocotyls and cotyledons, 3 types of tissue were selected in later subcultures: (a) callus tissue strains that produced buds, (b) embryogenic tissue, and (c) unorganized callus tissue, lacking any organogenic capacity. Pretreatment with 2,4-dichlorophenoxyacetic acid increased the number of explants which gave rise to bud forming and embryogenic tissue, but was not essential for morphogenesis. Somatic embryogenesis was confirmed by histological observation. Plantlets could be easily obtained by inducing adventitious roots on shoots, but spontaneous root development in somatic embryos was infrequent.
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Abbreviations
- BAP:
-
benzylaminopurine
- IAA:
-
indole-3-acetic acid
- 2,4-D:
-
dichlorophenoxyacetic acid
- IBA:
-
indole-3-butyric acid
References
Adachi T (1986) In: Buckwheat Research 1986. Proc 3rd Intern Symp on Buckwheat, Pulawy, Poland, pp 108–115
Ćulafić L, Budimir S, Vujičić R, Nešković M (1987) Plant Cell Tissue Organ Cult (in press)
De Jong H (1972) Field Crop Abstr 25: 389–396
Gamborg OL, Miller RA, Ojima K (1968) Exper Cell Res 50: 148–151
Kreft I (1983) In: Buckwheat Research 1983. Proc 2nd Intern Symp on Buckwheat, Miyazaki, Japan, pp 3–12
Maheswaran G, Williams EG (1984) Ann Bot 54: 201–211
Maheswaran G, Williams EG (1986) J Plant Physiol 124: 455–463
Nešković M, Srejović V, Vujičić R (1986) In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry 2, Crops I. Springer, Berlin Heidelberg New York, pp 579–593
Pomeranz Y, Robbins GS (1972) J Agr Food Chem 20: 270–274
Pretova A, Williams EG (1986) J Plant Physiol 126: 155–161
Ranch JP, Oglesby L, Zielinski AC (1985) In Vitro 21: 653–658
Srejović V, Nešković M (1981) Z Pflanzenphysiol 104: 37–42
Walker KA, Wendeln ML, Jaworski EG (1979) Plant Sci Lett 16: 23–30
Yamane Y (1974) Jap J Genet 49: 139–146
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Communicated by H. Lörz
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Nešković, M., Vujičić, R. & Budimir, S. Somatic embryogenesis and bud formation from immature embryos of buckwheat (Fagopyrum esculentum Moench.). Plant Cell Reports 6, 423–426 (1987). https://doi.org/10.1007/BF00272773
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DOI: https://doi.org/10.1007/BF00272773