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Analysis of rho mutability in Saccharomyces cerevisiae

I. Effects of mmc and pet-ts alleles

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Summary

Two additional types of nuclear determinants involved in the control of spontaneous mutability of rho in S. cerevisiae have been identified: mmc and the pet-ts 1, 2, 10, 52 and 53 genes.

These genes in their mutated recessive form increase at various extents the number of respiratory deficient cytoplasmic “petite” mutants accumulated.

The gene mmc does not affect the respiratory activity and is not temperature-dependent whereas the pet-ts genes determine at the non permissive temperature a respiratory deficient phenotypes even if they affect the mutability of rho at the permissve and at the non permissive temperature.

The data here reported suggest that a “replicative complex” exists for the mitochondrial DNA.

It is in the purpose of this paper to deal with the relative contrition that mmc and pet-ts gene products have in ensuring the fidelity of this “replicative complex”.

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References

  • Backhaus, B., Schweyen, R.J., Kaudewitz, F., Dujon, B.: On the formation of ϱ-petites in yeast: III Effect of temperature on transmission and recombination of mitochondrial markers and on ϱ-cell formation in temperature sensitive mutants of Saccharomyces cerevisiae. Mol. Gen. Genet. 161, 153–173 (1978)

    Google Scholar 

  • Bechmann, H., Krüger, M., Böker, E., Bandlow, W., Schweyen, R.J., Kaudewitz, F.: On the formation of ϱ-petites in yeast: II Effects of mutation tsm-8 on mitochondrial function and ϱ factor stability in Saccharomyces cerevisiae. Mol. Gen. Genet. 155, 41–51 (1977)

    Google Scholar 

  • Bolotin-Fukuhara, M., Faye, G., Fukuhara, H.: Temperature-sensitive respiratory-deficient mitochondrial mutations: isolation and genetic mapping. Mol. Gen. Genet. 152, 295–305 (1977)

    Google Scholar 

  • Burkl, G., Demmer, W., Holzner, H., Schweizer, E.: Temperature sensitive nuclear peptite mutants of Saccharomyces cerevisiae. In: Genetics, biogenesis and bioenergetis of mitochondria (Baudlow, W., Schweyen, R.J., Thomas, D.Y., Wolf, K., Kaudewitz, F., eds.), pp. 39–48. Berlin-New York: de Gruyter 1976

    Google Scholar 

  • Ephrussy, B., Hottinguer, H.: On an unstable cell state in yeast. Cold Spring Harbor Symp. Quant. Biol. 16, 75–85 (1951)

    Google Scholar 

  • Handwerker, A., Schweyen, R.J., Wolf, K., Kaudewitz, F.: Evidence for an extrakaryotic mutation affecting the maintenance of the rho factor in yeast. J. Bacteriol. 113, 1307–1310 (1973)

    Google Scholar 

  • Lea, D.E., Coulson, C.A.: The distribution of the numbers of mutants in bacterial populations. J. Genet. 49, 264–285 (1949)

    Google Scholar 

  • Louie, A., Boerner, P., Biron, C.A., Mason, T.L.: Mitochondria assembly in temperature sensitive mutants of S. cerevisiae. Fed. Proc. 34, 497 Abs 1556 (1975)

    Google Scholar 

  • Lusena, C.V., James, A.P.: Alterations in mitochondrial DNA of yeast which accompany genetically and enviromentally controlled changes in mutability. Mol. Gen. Genet. 144, 119–125 (1976)

    Google Scholar 

  • Magni, G.E., von Borstel, R.C.: Different rates of spontaneous mutation during mitosis and meiosis in yeast. Genetics 47, 1097–1108 (1962)

    Google Scholar 

  • Magni, G.F., von Borstel, R.C., Sora, S.: Mutagenic during meiosis and antimutagenic action during mitosis by 5-Aminoacridine in yeast. Mutat. Res. 1, 227–230 (1964)

    Google Scholar 

  • Marmiroli, N., Donnini, C., Restivo, F.M., Tassi, F., Puglisi, P.P.: Analysis of rho mutability in Saccharomyces cerevisiae. II. Role of the mitochondrial protein synthesis. Mol. Gen. Genet. 177, 589–595 (1980)

    Google Scholar 

  • Marmiroli, N., Vecli, A., Carboni, G., Puglisi, P.P.: Isoelectric focusing analysis of mitochondrial proteins in a temperature-sensitive petite mutants of Saccharomyces cerevisiae. In: Progress in isolectric focusing and isotachophoresis (Righetti, P.G., ed.), pp. 165–172. Amsterdam: North Holland Publishing Company 1975

    Google Scholar 

  • Moustacchi, E.: Evidence for nucleus indepentent steps in control of repair of mitochondrial damage. I. UV induction of the cytoplasmic “petite” mutation in UV sensitive nuclear mutants of Saccharomyces cerevisiae. Mol. Gen. Genet. 114, 50–58 (1972)

    Google Scholar 

  • Moustacchi, E.: Cytoplasmic “petite” induction in recombination-deficient mutants of Saccharomyces cerevisiae. J. Bacteriol. 115, 805–809 (1973)

    Google Scholar 

  • Ogur, M., St. John, R., Nagai, S.: Tetrazolium overlay technique for population studies of respiration deficiency in yeast. Science 125:928–929 (1957)

    Google Scholar 

  • Puglisi, P.P., Calzigna, L.: Un mutante petite nucleare termosensibile in S. cerevisiae. Atti Ass. Genet. Ital. 13, 291–292 (1968)

    Google Scholar 

  • Puglisi, P.P., Cremona, T.: Biochemical properties of a nuclear thermosensitive mutant of Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 39, 461–468 (1970)

    Google Scholar 

  • Sherman, F., Slonimski, P.P.: Respiration deficient mutants of yeast. II Biochemistry. Biochim. Biophys. Acta 90, 1–15 (1964)

    Google Scholar 

  • Slonimski, P.P., Tzagoloff, A.: Locazation in yeast mitochondrial DNA of mutations expressed in a deficiency of cytochrome oxidase and for coenzyme QH2-cytochrome-c-reductase. Eur. J. Biochem. 61, 27–41 (1976)

    Google Scholar 

  • Storm, E.M., Marmur, J.A.: A temperature-sensitive mitochondrial mutation of Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 64, 752–759 (1976)

    Google Scholar 

  • Sjostrand, F.S., Barajas, L.: A new model for mitochondrial membranes based on structural and on biochemical information. J. Ultrastruct. Res. 32, 293–306 (1970)

    Google Scholar 

  • Trembath, M.K., Monk, B.C., Kellerman, G.M., Linnane, A.W.: Biogenesis of mitochondria. 36. The genetic and biochemical analysis of a mitochondrially determined cold-sensitive oligomycin-resistant mutant of Saccharomyces cerevisiae with affected mitochondrial ATPase assembly. Mol. Gen. Genet. 141, 9–22 (1975a)

    Google Scholar 

  • Trembath, M.K., Monk, B.C., Kellerman, G.M., Linnane, A.W.: Biogenesis of mitochondria. 40. Phenotypic suppression of a mitochondrial mutation by a nuclear gene in Saccharomyces cerevisiae. Mol. Gen. Genet. 140, 333–337 (1975b)

    Google Scholar 

  • Tzagoloff, A., Foury, F., Akai, A.: Assembly of the mitochondrial membrane system XVIII. Genetic loci on mitochondrial DNA involved in cytochrome b biosynthesis. Mol. Gen. Genet. 149, 33–42 (1976)

    Google Scholar 

  • Weislogel, P.O., Butow, R.A.: Low temperature and chloramphenicol induction of respiratory deficiency in a cold sensitive mutant of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci US. A. 67, 52–58 (1970)

    Google Scholar 

  • Williamson, D.H.: The effect of environmental and genetic factors on the replication of mitochondrial DNA in yeast. In: Control of organelle development (Miller, P., ed.), Soc. Exp. Biol. 24, pp. 247–276. Cambridge: The University Press 1970

    Google Scholar 

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Authors and Affiliations

  1. Institute of Genetics, University of Parma, (Italy)

    N. Marmiroli, F. M. Restivo, C. Donnini, L. Bianchi & P. P. Puglisi

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  1. N. Marmiroli
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  2. F. M. Restivo
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  3. C. Donnini
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  4. L. Bianchi
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  5. P. P. Puglisi
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Communicated by F. Kaudewitz

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Marmiroli, N., Restivo, F.M., Donnini, C. et al. Analysis of rho mutability in Saccharomyces cerevisiae . Molec. Gen. Genet. 177, 581–588 (1980). https://doi.org/10.1007/BF00272667

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  • DOI: https://doi.org/10.1007/BF00272667

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