Abstract
Haploid tobacco (Nicotiana sylvestris) plants were inoculated with a yellow strain of tobacco mosaic virus (TMV-Flavum) and then exposed to 500 rads of acute gamma radiation. Leaf strips cultured on callus-inducing medium yielded two types of colonies: 1) yellow, virus-infected and 2) green, apparently healthy. Of the 3210 calli scored, approximately 5% were virus-free, and after regeneration, 0.2% were resistant at the plant stage. Later, adult plants, both TMV-resistant and TMV-susceptible, produced self-fertile, diploid flowers. Both seedling progeny and rooted cuttings from resistant stock plants showed resistance to TMV infection. This resistance was characterized by restricted virus multiplication and movement within the infected plant resulting in a 3–8 week delay in symptom expression.
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References
Behnke M (1979) Theoretical Applied Genetics 55:69–71
Behnke M (1980) Theoretical Applied Genetics 56:151–152
Carlson PS (1973) Science 180:1366–1368
Gengenbach BG, Green CE, Donovan, CM (1977) Proceedings of the National Academy of Science USA 74:5113–5117
Gooding GV, Herbert TT (1967) Phytopathology 57:1285
Ingram DS (1976) In: Heitefuss R, Williams PH (eds) Physiological plant pathology, Vol 4, Springer Verlag, Berlin Heidelberg New York pp 743–759
Kasperbauer NH, Collins GB (1972) Crop Science 12:98–101
Krishnamurthi M, Tlaskal, J (1974) Proceedings of the International Society of Sugarcane Technologists 15:130–137
Linsmaier EM, Skoog F (1965) Physiologia Plantarum 18:100–127
Lucas GB (1975) In: Diseases of Tobacco, 3rd Edition. HE Parker and Sons, Printers, Fuquay-Varina, NC 27526, p 444
Matern U, Strobel G, Shepard J (1978) Proceedings of the National Academy of Science USA 75:4935–4939
Murakishi HH, Carlson PS (1981) Abstract No. 64 of American Phytopathological Society Annual Meeting
Nickel LG (1977) Crop Science 17:717–719
Shepard JF (1980) In: Rubenstein I, Gengenbach B, Phillips R, Green C (eds) Genetic improvement of crops: emergent techniques, University of Minnesota Press, Minneapolis, pp 185–219
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Journal Paper No. 10138 of the Michigan Agricultural Experiment Station.
This work was supported in part by U.S.D.A. grant no. 79-59-2261-1-1-351-1.
We thank Drs. M. Daub, R. Griesbach, and J. Hunsperger for helpful suggestions.
The excellent technical assistance of Ms. Brenda Floyd and Sara Stadt is acknowledged.
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Murakishi, H.H., Carlson, P.S. In vitro selection of Nicotiana sylvestris variants with limited resistance to TMV. Plant Cell Reports 1, 94–97 (1982). https://doi.org/10.1007/BF00272361
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DOI: https://doi.org/10.1007/BF00272361