Summary
We have isolated and sequenced an anther-specific gene from sunflower which encodes an 800-nucleotide transcript detectable in the peripheral anther cells. It contains an intron of 2615 bp, which separates the first exon (77 bp) coding for a putative signal peptide of 21 amino acids, from the second exon (563 bp) coding for a 100 amino acid polypeptide. The 5′ and 3′ untranslated regions comprise respectively 13 and 264 bp. The SF2 gene is present in the sunflower genome in several copies, all or most of which contain a closely related intron.
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References
Benton WD, Davis RW (1977) Screening lambda gt recombinant clones by hybridization to single plaques. Science 196:180–182
Birnboim HC, Doly J (1979) A rapid extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Birnstiel M, Busslinger M, Strub K (1985) Transcription termination and 3′ processing: the end is in site! Cell 41:349–359
Breathnach R, Chambon P (1981) Organization and expression of eukaryotic split genes coding for proteins. Annu Rev Biochem 50:349–383
Brown SM, Crouch ML (1990) Characterization of a gene family abundantly expressed in Oenothera organensis pollen that shows sequence similarity to polygalacturonidase. Plant Cell 2:263–274
Davis RW, Botstein D, Roth JR (1980) Advanced bacterial genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: Version 2. Plant Mol Biol Reporter 1:19–21
Domon C, Evrard JL, Herdenberger F, Pillay DTN, Steinmetz A (1990) Nucleotide sequence of two anther-specific cDNAs from sunflower (Helianthus annuus L). Plant Mol Biol 15:643–646
Dunn IS, Blattner F (1987) Charons 36 to 40: multi enzyme, high capacity, recombination deficient vectors with polylinkers and polystuffers. Nucleic Acids Res 15:2677–2698
Evrard JL, Jako C, Saint-Guily A, Weil JH, Kuntz M (1991) Anther-specific, developmentally regulated expression of genes encoding a new class of proline-rich proteins in sunflower. Plant Mol Biol 16:271–281
Feinberg AP, Vogelstein S (1984) A technique for radiolabelling DNA fragments to high specific activity. Anal Biochem 137:266–267
Georgiev O, Birnstiel ML (1985) The conserved CAAGAAAGA spacer sequence is an essential element for the formation of 3′ termini of the sea urchin H3 historic mRNA by RNA processing. EMBO J 4:481–489
Goodall GJ, Filipowicz W (1989) The AU-rich squences present in the introns of plant nuclear pre-mRNAs are required for splicing. Cell 58:473–483
Gualberto JM, Wintz H, Weil JH, Grienenberger JM (1988) The genes coding for subunit 3 of NADH dehydrogenase and for ribosomal protein S12 are present in the wheat and maize mitochondrial genomes and are cotranscribed. Mol Gen Genet 215:118–127
Hanson DD, Hamilton DA, Travis JL, Bashe DM, Mascarenhas JP (1989) Characterization of a pollen-specific cDNA clone from Zea mays and its expression. Plant Cell 1:173–179
Hawkins JD (1988) A survey on intron and exon lengths. Nucleic Acids Res 16:9893–9908
Herdenberger F, Evrard JL, Kuntz M, Tessier LH, Klein A, Steinmetz A, Pillay DTN (1990) Isolation of flower-specific cDNA clones from sunflower (Helianthus annuus L.) Plant Sci 69:111–122
Kaiser K, Murray N (1985) In: Glover DM (ed) DNA cloning, vol 1. IRL Press, Oxford, pp 1–47
Kamalay JC, Goldberg RB (1980) Regulation of structural gene expression in tobacco. Cell 19:935–946
Kamalay JC, Goldberg RB (1984) Organ-specific nuclear RNAs in tobacco. Proc Natl Acad Sci USA 81:2801–2805
Kay SA, Keith B, Shinozaki K, Chye ML, Chua NH (1989) The rice phytochrome gene: structure, autoregulated expression, and binding of GT-1 to a conserved site in the 5′ upstream region. Plant Cell 1:351–360
Koltunow AM, Truettner J, Cox C, Wallroth M, Goldberg RB (1990) Different temporal and spatial gene expression patterns occur during anther development. Plant Cell 2:1201–1224
Mascarenhas JP (1990) Gene activity during pollen development. Annu Rev Plant Physiol 41:317–338
McCormick S, Twell D, Wing R, Ursin V, Yamagushi J, Larabell S (1989) Anther-specific genes: molecular characterization and promoter analysis in transgenic plants. In: Lord E, Bernier G (eds) Plant reproduction: From floral induction to pollination. American Society of Plant Physiologists Symposium Series, vol 1, pp 128–135
Nielsen NC, Dickinson CD, Cho T-J, Thanh VH, Scallon BJ, Fischer RL, Sims TL, Drews GN, Goldberg RB (1989) Characterization of the glycinin gene family in soybean. Plant Cell 1:313–328
Proudfoot N (1991) Poly(A) signals. Cell 64:671–674
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Seurinck J, Truettner J, Goldberg RB (1990) The nucleotide sequence of an anther-specific gene. Nucleic Acids Res 18:3403
Smith AG, Gasser CS, Budelier KA, Fraley RT (1990) Identification and characterization of stamen and tapetum-specific genes from tobacco. Mol Gen Genet 222:9–16
Stinson JR, Eisenberg AJ, Willing RP, Pe ME, Hanson DD, Mascarenhas JP (1987) Genes expressed in the male gametophyte of flowering plants and their isolation. Plant Physiol 83:442–447
Twell D, Wing R, Yamagushi J, McCormick S (1989) Isolation and expression of an anther-specific gene from tomato. Mol Gen Genet 247:240–245
Ursin VM, Yamagushi J, McCormick S (1989) Gametophytic and sporophytic expression of anther-specific genes in developing tomato anthers. Plant Cell 1:727–736
van Tunen AJ, Mur LA, Brouns GS, Rienstra JD, Koes RE, Mol JNM (1990) Pollen and anther-specific promoters from Petunia: tandem promoter regulation of the chiA gene. Plant Cell 2:393–401
von Heijne G (1983) Patterns of amino acids near signal-sequence cleavage sites. Eur J Biochem 133:17–21
Zhang H, Scholl R, Browse R, Somerville C (1988) Double stranded DNA sequencing as a choice for DNA sequencing. Nucleic Acids Res 16:1220
Zuker M, Stiegler P (1981) Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information. Nucleid Acids Res 9:133–148
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Communicated by J. Schell
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Domon, C., Evrard, JL., Pillay, D.T. et al. A 2.6 kb intron separates the signal peptide coding sequence of an anther-specific protein from the rest of the gene in sunflower. Molec. Gen. Genet. 229, 238–244 (1991). https://doi.org/10.1007/BF00272161
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DOI: https://doi.org/10.1007/BF00272161