Summary
Allelism tests between the standard Uq element (Uq1) and five newly activated germinal Uq elements (Uq2, Uq3, UQ4, Uq5, and Uq6) demonstrate that these new Uq elements are independent of Uq1. Gametes that either contain one Uq or various combinations of two different and phenotypically distinguishable Uq elements, have been constructed either with or without the a-ruq reporter allele. Genetic analyses of the progenies of the gametes (using the standard a-ruq tested line as the other parent) have indicated that (i) each Uq element, when present alone, has the capacity to express full activity except when a secondary transposition or loss of activity has occurred; (ii) all five new Uq elements are independent of Uq1 with respect to transposition activity; and (iii) these newly originated Uqs are clustered on one linkage group. Uq2 is allelic to Uq4, and Uq3 is allelic to Uq5, whereas Uq6 is linked to both allelic pairs. A putative linkage map of these Uq elements is presented. In reciprocal crosses there is a striking difference in phenotypic segregation of Uq; when transmitted via the male parent Uq loses full expression capacity.
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Burr B, Burr FA, Thompson KH, Albertson MC, Stuber CW (1988) Gene mapping with recombinant inbreds in maize. Genetics 118:519–526
Caldwell EEO, Peterson PA (1989) Diversity of transposable element interactions: The Uq transposable element system in maize controls for c-m mutants exhibiting unique responses to Uq13. Maydica 34:89–105
Cormack JB, Cox DF, Peterson PA (1988) Presence of the transposable element Uq in maize breeding material. Crop Sci 28:941–944
Dellaporta SL, Wessler S (1984) Ds1 element at the waxy locus. In: Ritcey E, Brown D, Kirk A (eds) Cold Spring Harbor Laboratory Annual Report, 1984. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 107–108
Friedemann PD, Peterson PA (1982) The Uq controlling element system in maize. Mol Gen Genet 187:19–29
Johns MA, Fuerstenberg SI, Hennelly CA (1990) Non-random chromosomal distribution of Ac-like sequences in inbred maize. Genet Res 55:71–80
Karazawa M, Peterson PA (1987) Test for presence of controlling elements in maize populations. Maize Genet Coop Newslett 61:4
McClintock B (1950) Mutable loci in maize. Carnegie Inst Washington Yearb 49:159–167
McClintock B (1958) The suppressor-mutator system of control of gene action in maize. Carnegie Inst Washington Yearb 57:414–429
Muller-Neumann M, Yoder JI, Starlinger P (1984) The DNA sequence of the transposable element Ac of Zea mays L. Mol Gen Genet 198:19–24
Nowick EM, Peterson PA (1981) Transposition of the Enhancer controlling element system in maize. Mol Gen Genet 183:440–448
Pan Y-B, Peterson PA (1987) A survey of active Uq element in maize inbred lines by the use of c-ruq rester. Maize Genet Coop Newslett 61:5
Pan Y-B, Peterson PA (1988) Spontaneous activation of quiescent Uq transposable elements during endosperm development in Zea mays. Genetics 119:457–464
Pan Y-B, Peterson PA (1989a) Tagging of a maize gene involved in kernel development by an activated Uq transposable element. Mol Gen Genet 219:324–327
Pan Y-B, Peterson PA PA (1989b) Uq 10aa-SS second 5-aza-2′deoxycytidine induced Uq mutant. Maize Genet Coop Newslett 63:19–20
Pan Y-B, Peterson PA (1991) Spontaneous germinal activation of quiescent Uq transposable elements in Zea mays L. Genetics, in press
Pereira A, Peterson PA (1985) Origin and diversity of mutants controlled by the Uq transposable element system in maize. Genet Res 46:219–236
Peterson PA (1976) Basis for the diversity of states of controlling elements in maize. Mol Gen Genet 149:5–21
Peterson PA (1985) Virus-induced mutations in maize: on the nature of stress-induction of instable loci. Genet Res 46:207–217
Peterson PA (1986) Mobile elements in maize: a force in evolutionary and plant breeding process. In: Gustafson JB, Stebbins GL, Ayala FJ (eds) Genetics, development, and evolution. Plenum, New York, pp 47–78
Peterson PA (1987) Mobile elements in plants. CRC Crit Rev Plant Sci 6:105–208
Peterson PA, Friedemann PD (1983) The Ubiquitous controlling element system and its distribution in assorted maize testers. Maydica 28:213–249
Peterson PA, Salamini F (1986) A search for active mobile elements of Iowa stiff stalk synthetic maize population and some derivatives. Maydica 31:163–172
Pohlman RF, Fedoroff NV, Messing J (1984) The nucleotide sequence of the maize controlling element Activator. Cell 37:635–643
Sprague GF (1986) Mutability in the a-ruq, Uq system in maize. Maydica 31:17–30
Sprague GF, McKinney HH (1966) Aberrant Ratio: an anomaly in maize associated with virus infection. Genetics 54:1287–1296
Sutton WD, Gerlach WL, Schwartz D, Peacock WJ (1984) Molecular analysis of Ds controlling element mutations at the Adh locus of maize. Science 223:1265–1268
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Pan, YB., Peterson, P.A. Newly activated germinal Uq elements in maize are clustered on one linkage group independently of the standard Uq element. Molec. Gen. Genet. 229, 161–174 (1991). https://doi.org/10.1007/BF00272152
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DOI: https://doi.org/10.1007/BF00272152