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Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii

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Abstract

Inner membranes of Rhizobium leguminosarum biovars viciae, phaseoli, and trifolii synthesized a heterogenous family of neutral cyclic β-(1,2)-glucans in a reaction system that used oligosaccharide intermediates covalently bound to a large protein. This glucoprotein showed a slightly lower mobility on SDS-polyacrylamide gels (apparent mol. mass of 320 kDa) than the β-(1,2)-glucan intermediate protein of Rhizobium meliloti. In vivo pulse-label experiments with growing cells of R. leguminosarum biovar trifolii RS800 using radioactive glucose showed that few species of cyclic β-(1,2)-glucans were synthesized and up to 30% were substituted with charged non-glycosidic residues, probably sn-1-phosphoglycerol.

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Author notes

  1. Carlos Semino

    Present address: Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, USA

Authors and Affiliations

  1. Instituto de Investigaciones Bioquímicas “Fundación Campomar”, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires and CONICET, Avenida Patricias Argentinas 435, 1405, Buenos Aires, Argentina

    Olga A. Castro, Angeles Zorreguieta, Carlos Semino & Luis Ielpi

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  1. Olga A. Castro
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  2. Angeles Zorreguieta
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  3. Carlos Semino
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  4. Luis Ielpi
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Correspondence to Angeles Zorreguieta.

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Castro, O.A., Zorreguieta, A., Semino, C. et al. Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii . Arch. Microbiol. 163, 454–462 (1995). https://doi.org/10.1007/BF00272135

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  • DOI: https://doi.org/10.1007/BF00272135

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