Abstract
A total of 19 different species belonging to the genera Asperula, Galium, Rubia and Sherardia were taken into cell culture. All species, differentiated plants as well as tissue cultures, produced anthraquinones in differing yields. Cells were grown in a basal medium containing 7 differently substituted phenoxyacetic acids, as well as 1-naphthaleneacetic acid, all at 10−5 M concentration. The effectors supporting highest pigment production in each culture were selected and, in the presence of the selected effector, the sucrose content of the medium was then varied from 1 to 14%. Anthraquinone formation was thus optimized for each individual species, but no general pattern, either of effector quality or of sucrose concentration, emerged. In 17 out of 19 cases secondary product formation in optimized cell cultures surpassed that of differentiated plants. The highest anthraquinone yield was observed with Galium verum (1.7 g/l) and the highest concentration achieved with Rubia fruticosa (20% of dry weight).
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
1-naphthaleneacetic acid
- PAA:
-
phenoxyacetic acid
- dwt:
-
dry weight
References
Bauch J, Leistner E (1978) Planta medica 33: 105.
Dansette P (1972) Etude sur la Biosynthese des Naphthoquinones Vegetales et bacteriennes, Dissertation, Paris.
Fujita Y, Hara Y, Suga C, Morimoto T (1981) Plant Cell Rep. 1: 61.
Fujiwara A (ed.) (1982) Plant tissue culture 1982, Japanese Association for plant tissue culture, Tokyo.
Inoue K, Nayeshiro H, Inouye H, Zenk MH (1981) Phytochemistry 20: 1963.
Kempter G (1971) Organisch-chemisches Praktikum. Unitext Braunschweig.
Mantell SH, Pearson DW, Hazell LP, Smith H (1983) Plant Cell Rep. 2: 73.
Thomson RH (1971) Naturally Occurring Quinons. Academic Press London, New York.
Zenk MH (1978) in Thorpe TA (ed.): Frontiers of Plant Tissue Culture. International Association of Plant Tissue Culture Calgary, pp 1–13.
Zenk MH, Schulte U, El-Shagi H (1984) Naturwissenschaften. In press.
Zenk MH, El-Shagi H, Schulte U (1975) Planta medica Suppl 75.
Zenk MH, El-Shagi H, Arens H, Stöckigt J, Weiler E, Deus B (1977) Plant Tissue culture and it's biotechnological application (Barz et al. eds.) Springer Verlag, Berlin, Heidelberg, New York, pp 27.
Suzuki H, Matsumoto T, Obi Y (1982) in Fujiwara A (ed.): Plant Tissue Culture 1982, Japanese Association for Plant Tissue Culture, Tokyo, pp 285.
Gamborg OL, Miller RA, Ojima K (1968) Exptl. Cell Res. 50: 151.
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Communicated by K. Hahlbrock
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Schulte, U., El-Shagi, H. & Zenk, M.H. Optimization of 19 Rubiaceae species in cell culture for the production of anthraquinones. Plant Cell Reports 3, 51–54 (1984). https://doi.org/10.1007/BF00270970
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DOI: https://doi.org/10.1007/BF00270970