Summary
Spores of heterothallic diploid cells of Saccharomyces cerevisiae had neither a nor α agglutination substance in either cell wall or cytoplasmic fraction; they, however, showed selfagglutination not caused by sex-specific agglutination substances. Meanwhile, practically no sexual agglutination was detected during germination and outgrowth of the spores; it arose after emergence of the first buds and progressed with incubation time. Its ability increased gradualy until the first bud emergence and rapidly thereafter. a and α agglutination substances were detected in both cell wall and cytoplasmic fractions of cells from an 8h-old spore culture. Only germinated spores with buds had the ability to produce and to respond to the a pheromone.
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References
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28P350–356
Haber JE, Halvorson HO (1975) Methods in sporulation and germination of yeasts. In: Prescott DM (ed) Methods in cell biology, vol 11. Academic Press, New York, pp 45–69
Hagiya M, Yoshida K, Yanagishima N (1977) The release of sexspecific substances responsible for sexual agglutination from haploid cells of Saccharomyces cerevisiae. Exp. Cell Res 104:263–272
Norris JR, Swain H (1971) Staining bacteria. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 5A. Academic Press, London, pp 105–134
Rousseau P, Halvorson HO (1969) Preparation and storage of single spores of Saccharomyces cerevisiae. J Bacteriol 100:1426–1427
Sakai K, Yanagishima N (1971) Mating reaction in Saccharomyces cerevisiae. I. Cell agglutination related to mating. Arch Mikrobiol 75:260–265
Sakai K, Yanagishima N (1972) Mating reaction in Sacchromyces cerevisiae. II. Hormonal regulation of agglutinability of a type cells. Arch Mikrobiol 84:191–198
Shimoda C, Yanagishima N (1974) Mating reaction in Saccharomyces cerevisiae. VI. Effect, of 2-deoxyglucose on conjugation. Plant Cell Physiol 15:767–778
Shimoda C, Yanagishima N (1975) Mating reaction in Saccharomyces cerevisiae. VIII Mating-type-specific substances responsible for sexual cell agglutination. Antonie van Leeuwenhoek 41:521–532
Shimoda C, Yanagishima N, Sakurai A, Tamura S (1976) Mating reaction in Saccharomyces cerevisiae. IX Regulation of sexual cell agglutinability of a type cells by a sex factor produced by α type cells. Arch Microbiol 108:27–33
Tohoyama H, Hagiya M, Yoshida K, Yanagishima N (1979) Regulation of the production of the agglutination substances responsible for sexual agglutination in Saccharomyces cerevisiae: Changes associated with conjugation and temperature shift. Mol Gen Genet 174:269–280
Yanagishima N, Nakagawa Y (1980) Mutants inducible for sexual agglutinability in Saccharomyces cerevisiae. Mol Gen Genet 178:241–251
Yanagishima N, Yoshida K (1981) Sexual interactions in Saccharomyces cerevisiae with special reference to the regulation of sexual agglutinability. In: O'Day DH, Horgen PA (eds) Sexual interactions in eukaryotic microbes. Academic Press, New York, pp 261–295
Yoshida K, Hagiya M, Yanagishima N (1976) Isolation and purification of the sexual agglutination substance of mating type a cells in Saccharomyces cerevisiae. Biochem Biophys Res Commun 71:1085–1094
Yoshida K, Yanagishima N (1978) Intra-and intergeneric mating behaviour of ascosporogenous yeasts. I. Quantitative analysis of sexual agglutination. Plant Cell Physiol 19:1519–1533
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Communicated by F. Kaudewitz
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Tohoyama, H., Yanagishima, N. Changes in sexual agglutination ability during the formation of vegetative cells from spores in Saccharomyces cerevisiae . Molec. Gen. Genet. 183, 205–208 (1981). https://doi.org/10.1007/BF00270618
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DOI: https://doi.org/10.1007/BF00270618