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Isolation of plasmids carrying the arginine repressor gene argR of Escherichia coli K12

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Summary

A plasmid carrying the arginine repressor gene (argR) of Escherichia coli was obtained out of the Clarke and Carbon colony bank which contains clones of individual ColE1 plasmids carrying fragments of the E. coli chromosome. A shortened derivative of this plasmid was isolated and analyzed by restriction mapping and by cloning fragments of it into plasmid pBR322. The argR gene was located on a 2 kb fragment.

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References

  • Bolivar, F., Rodriguez, R.L., Greene, P.J., Betlach, M.L., Heyneker, H.L., Boyer, H.W.: Construction and characterization of new cloning vehicles II: — a multipurpose cloning system. Gene 2, 95–113 (1977)

    Google Scholar 

  • Clarke, L., Carbon, J.: A colony bank containing synthetic ColE1 hybrid plasmids representative of the entire Escherichia coli genome. Cell 9, 91–99 (1976)

    Google Scholar 

  • Cunin, R., Kelker, N., Boyen, A., Yang, H., Zubay, G., Glansdorff, N., Maas, W.: Involvement of arginine in in vitro repression of arginine genes C. B, and H in Escherichia coli K12. Biochim. Biophys. Res. Comm. 69, 377–382 (1976)

    Google Scholar 

  • Eckhardt, T.: Use of argA-lac fusions to generate lambda argA-lac bacteriophages and to determine the direction of argA transcription in Escherichia coli. J. Bacteriol. 132, 60–66 (1977)

    Google Scholar 

  • Eckhardt, T.: A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria. Plasmid 1, 584–588 (1978)

    Google Scholar 

  • Eckhardt, T., Leisinger, T.: Isolation and characterization of mutants with a feedback resistent N-acetylglutamate synthase in Escherichia coli K12. Mol. Gen. Genet. 138, 225–232 (1975)

    Google Scholar 

  • Kelker, N., Maas, W., Yang, H.L., Zubay, G.: In vitro synthesis and repression of argininosuccinase in Escherichia coli K-12: partial purification of the arginine repressor. Mol. Gen. Genet. 144, 17–20 (1976)

    Google Scholar 

  • Meagher, R., Tait, R., Betlach, M., Boyer, H.: Protein expression in E. coli minicells by recombinant plasmids. Cell 10, 521–536 (1977)

    Google Scholar 

  • Miller, J.: Experiments in molecular genetics. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory, 1972

    Google Scholar 

  • Oka, A.: Fine cleavage map of a small colicin E1 plasmid carrying genes responsible for replication and colicin immunity. J. Bacteriol. 133, 916–924 (1978)

    Google Scholar 

  • Oka, A., Nomura, N., Morita, M., Sugisaki, H., Sugimoto, K., Takanami, M.: Nucleotide sequence of small ColE1 derivatives: Structure of the region essential for autonomous replication and colicin E1 immunity. Mol. Gen. Genet. 172, 151–159 (1979)

    Google Scholar 

  • Sutcliffe, J.: pBR322 restriction map derived from DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucleic Acids Res. 5, 2721–2728 (1978a)

    Google Scholar 

  • Sutcliffe, J.: Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symp. Quant. Biol. 43, 77–90 (1978b)

    Google Scholar 

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Communicated by W. Arber

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Eckhardt, T. Isolation of plasmids carrying the arginine repressor gene argR of Escherichia coli K12. Molec. Gen. Genet. 178, 447–452 (1980). https://doi.org/10.1007/BF00270498

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