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Virulent mutants of temperate Rhizobium meliloti phage 16-3: Loci avirC and avirT, and increased recombination

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Summary

Two loci avirC and avirT were identified on the genome of temperate Rhizobium meliloti phage 16-3. Virulence (immunity insensitivity) required mutations of both loci. Locus avirC mapped at the boundary of the C (repressor) cistron and the silent chromosomal arm, locus avirT mapped at the other side of C cistron close to the nearest early mutation ts5124. One virulent mutant had an increased recombination ability.

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References

  • Dallmann, G., Orosz, L., Sain, B.: Restriction mapping of DNA of temperate Rhizobium meliloti phage 16-3: Comparison of genetic and physical maps indicates a long, genetically silent chromosomal arm. Mol. Gen. Genet. 176, 439–448 (1979)

    Google Scholar 

  • Ephrussi-Taylor, H., Sicard, A.M., Kamen, R.: Genetic recombination in DNA-induced transformation of Pneumococcus. I. The problem of relative efficiency of transforming factors. Genetics 51, 455–476 (1965)

    Google Scholar 

  • Hopkins, N., Ptashne, M.: Genetics of virulence. In: The bacteriophage lambda (A.D. Hershey, ed.), pp. 571–574. Cold Spring Harbor: Cold Spring Harbor Laboratory 1971

    Google Scholar 

  • Horiuchi, T., Koga, H., Inokuchi, H., Tomizawa, J.: Lambda phage mutants insensitive to temperature-sensitive repressor. I. Isolation and genetic analysis of weak-virulent mutants. Mol. Gen. Genet. 104, 51–58 (1969)

    Google Scholar 

  • Kondorosi, Á., Kiss, G., Forrai, T., Vincze, É., Bánfalvi, Zs.: Circular linkage map of Rhizobium meliloti chromosome. Nature 268, 525–527 (1977)

    Google Scholar 

  • Lam, S.T., Stahl, M.M., McMilin, K.D., Stahl, F.W.: Rec-mediated recombinational hot spot activity in bacteriophage lambda. II. A mutation which causes hot spot activity. Genetics 77, 425–433 (1974)

    Google Scholar 

  • Ördögh, F., Szende, K.: Temperate bacteriophages isolated from Rhizobium meliloti. Acta Microbiol. Acad. Sci. Hung. 7, 65–71 (1961)

    Google Scholar 

  • Olasz, F., Dallmann, G., Orosz, L.: Mutants of phage 16-3 of Rhizobium meliloti altered in genetic recombination. Acta Microbiol. Acad. Sci. Hung. (in press) (1980)

  • Orosz, L.: Methods for analysis of the C cistron of temperate phage 16-3 of Rhizobium meliloti. Genetics (in press) (1980)

  • Orosz, L., Sik, T.: Genetic mapping of Rhizobiophage 16-3. Acta Microbiol. Acad. Sci. Hung. 17, 185–194 (1970)

    Google Scholar 

  • Orosz, L., Rostás, K., Hotchkiss, R.D.: A comparison of two point, three point and deletion mapping in the C cistron of Rhizobium phage 16-3, and an explanation of the recombination pattern. Genetics (in press) (1980)

  • Orosz, L., Sváb, Z., Kondorosi, A., Sik, T.: Genetic studies on Rhizobiophage 16-3. I. Genes and functions on the chromosome. Mol. Gen. Genet. 125, 341–350 (1973)

    Google Scholar 

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Communicated by W. Arber

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Dallmann, G., Olasz, F. & Orosz, L. Virulent mutants of temperate Rhizobium meliloti phage 16-3: Loci avirC and avirT, and increased recombination. Molec. Gen. Genet. 178, 443–446 (1980). https://doi.org/10.1007/BF00270497

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  • DOI: https://doi.org/10.1007/BF00270497

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