Summary
The cytology of transfer diploidy produced in R. lupini by conjugation has been investigated. Since merodiploid donor strains transfer the diploid region as one transfer unit it is concluded that in merodiploid strains the two homologous regions are inserted in tandem sequence.
It has been discussed that the segregation patterns of diploid clones indicate that in this conjugation system the exogenote insertion always occurs in the zygote after transfer and before recombination.
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Anderson, R.P., Miller, C.G., Roth J.R.: Tandem duplications of the histidine operon observed following generalized transduction in Salmonella typhimurium. J. molec. Biol. 105, 201–218 (1976)
Anderson, R.P., Roth, J.R.: Tandem genetic duplications in phage and bacteria. Ann. Rev. Microbiol. 31, 473–505 (1977)
Anderson, T.F.: Recombination and segregation in Escherichia coli. Cold Spr. Harb. Symp. quant. Biol. 23, 47–58 (1958)
Audit, C., Anagnostopoulos, C.: Studies on the size of the diploid region in Bacillus subtilis merozygotes from strains carrying the trp E26 mutation. Molec. gen. Genet. 137, 337–351 (1975)
Heumann, W.: Conjugation in starforming Rhizobium lupini. Molec. gen. Genet. 102, 132–144 (1968)
Heumann, W., Pühler, A., Wagner, Erika: The two transfer regions of the Rhizobium lupini conjugation. I. Fertility factor elimination and one way transfer. Molec. gen. Genet. 113, 308–315 (1971)
Heumann, W., Pühler, A., Wagner, Erika: The two transfer regions of the Rhizobium lupini conjugation. II. Genetic characterization of the transferred chromosomal segments. Molec. gen. Genet. 126, 267–274 (1973)
Heumann, W., Springer, R.: Formation of merodiploid clones by conjugation in Rhizobium lupini. Molec. gen. Genet. 150, 73–79 (1977)
Hill, C.W., Combriato, G.: Genetic duplications induced at very high frequency by ultraviolet irradiation in Escherichia coli. Mol. gen. Genet. 127, 197–214 (1973)
Hill, C.W., Schiffer, D., Berg, P.: Transduction of merodiploidy: Induced duplication of recipient genes. J. Bact. 99, 274–478 (1969)
Johnson, E.M., Craig, W.G. Jr., Wohlhieter, J.A., Lazere, Janet R., Synenki, R.M., Baron, L.S.: Conservation of Salmonella typhimurium deoxyribonucleic acid in partially diploid hybrids of Escherichia coli. J. Bact. 115, 629–634 (1973)
Johnson, E.M., Placek, B.P., Snellings, N.J., Baron, L.S.: Conservation of Salmonella typhimurium deoxyribonucleic acid by chromosomal insertion in a partially diploid Escherichia coli hybrid. J. Bact. 123, 1–6 (1975)
Kashmiri, S.V.S., Hotchkiss, R.D.: Evidence of tandem duplication of genes in a merodiploid region of Pneumococcal mutants resistant to sulfonamide. Geneties 81, 21–31 (1975)
Leavitt, R.W., Wohlhieter, J.A., Johnson, E.M., Olson, G.E., Baron, L.S.: Isolation of circular deoxyribonucleic acid from Salmonella typhosa hybrids obtained from matings with Escherichia coli Hfr donors. J. Bact. 108, 1345–1357 (1971)
Lederberg, J.: Conjugal pairing in Escherichia coli. J. Bact. 71, 497–498 (1956)
Lederberg, J.: Sibling recombinants in zygote predigrees of Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 43, 1060–1065 (1957)
Lotan, D., Yagil, E., Bracha, M.: Bacterial conjugation: An analysis of mixed recombinant clones. Genetics 72, 381–391 (1972)
Ou, J.T.: Recombinant clone heterogeneity in Escherichia coli conjunction: Effect of pH and partially replicated recipient deoxyribonucleic acid. Genetics 80, 401–419 (1975)
Stodolsky, M.: Recipient gene duplication during generalized transduction. Genetics 78, 809–822 (1974)
Straus, D.S.: Induction by mutagens of tandem gene duplications in the gly S region of the Escherichia coli Genetics 78, 823–830 (1974)
Straus, D.S., Hoffmann, G.R.: Selection for a large genetic duplication in Salmonella thyphimurium. Genetics 80, 227–237 (1975)
Vierny, C. Janet, Anagnostopoulos, C.: Induction and transmission of a merodiploid condition near the terminal area of the chromosome of Bacillus subtilis. Genetics 81, 437–458 (1975)
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Communicated by F. Kaudewitz
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Springer, R., Heumann, W. Genetical analysis of merodiploidy in Rhizobium lupini . Molec. Gen. Genet. 165, 57–63 (1978). https://doi.org/10.1007/BF00270376
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DOI: https://doi.org/10.1007/BF00270376