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Plant regeneration from callus cultures of Durum and emmer wheat

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Abstract

Callus cultures were initiated from isolated mature embryos of Triticum turgidum L. Thell ssps durum and dicoccum on a basal medium supplemented with 2,4-D, 2,4,5-Cl3POP or 2,4-D+CM. Shoot bud regeneration was observed on 2,4,5-Cl3POP medium. In both the cultivars of durum, further development of shoot buds occurred on transfer of tissues to basal medium whereas in dicoccum basal medium supplemented with coconut milk or coconut milk with NAA (0.2 mg/l) was necessary. The regenerated shoot buds were induced to root on basal medium supplemented with NAA. The in vitro obtained plants were transferred to soil and successfully grown to maturity. Chlorophyll variants were observed among the regenerated plants of dicoccum.

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Abbreviations

BA:

benzyladenine

CM:

coconut milk

2,4-D:

2,4-dichlorophenoxyacetic acid

2,iP:

6-γ-γ-dimethylallylamine purine

IAA:

indoleacetic acid

NAA:

α-naphthalene acetic acid

Kn:

kinetin

2,4,5-Cl3POP:

2,4,5-trichlorophenoxypropionic acid

MS:

modified Murashige and Skoog's medium

RH:

relative humidity

Z:

zeatin

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Authors and Affiliations

  1. Plant Morphogenesis and Tissue Culture Section, Bio-Organic Division, Bhabha Atomic Research Centre, 400 085, Bombay, India

    Susan Eapen & P. S. Rao

Authors
  1. Susan Eapen
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  2. P. S. Rao
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Eapen, S., Rao, P.S. Plant regeneration from callus cultures of Durum and emmer wheat. Plant Cell Reports 1, 215–218 (1982). https://doi.org/10.1007/BF00270239

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  • DOI: https://doi.org/10.1007/BF00270239

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