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Secretion kinetics of hydroxyproline-containing macromolecules in carrot root discs

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Abstract

Short-term pulse-chase studies using radioactive L-proline on carrot tissue support the classical endomembrane route for secretory proteins. Labelled hydroxyproline residues were first detectable in fractions containing the endoplasmic reticulum (ER) after a 5 min pulse. Upon chase-out this fraction looses and, initially, the Golgi apparatus (GA) fraction gains radioactivity. Unlike ER and GA fractions which show chase-out characteristics a plasma membrane (PM) containing-fraction reveals retention of some of the radioactivity.

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Authors and Affiliations

  1. Abteilung Cytologie des Pflanzenphysiologischen Instituts der Universität Göttingen, Untere Karspüle 2, D-3400, Göttingen, Germany

    D. G. Robinson & R. Glas

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  1. D. G. Robinson
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  2. R. Glas
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Robinson, D.G., Glas, R. Secretion kinetics of hydroxyproline-containing macromolecules in carrot root discs. Plant Cell Reports 1, 197–198 (1982). https://doi.org/10.1007/BF00270233

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  • DOI: https://doi.org/10.1007/BF00270233

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