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High frequency of fusion induced in freely suspended protoplast mixtures by polyethylene glycol and dimethylsulfoxide at high pH

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Abstract

Fusion of freely suspended protoplast mixtures (hypocotyl protoplasts of Brassica napus mixed with mesophyll protoplasts of either B. campestris or Nicotiana plumbaginifolia) was induced by a solution containing 10% polyethylene glycol, 10% dimethyl-sulfoxide and 0.1M glycine-NaOH buffer (pH 10.0). The fusion products represented 15 to 17 percent of the surviving cells. More than 50% of the fusion products divided within two days after fusion, indicating that the fusion procedure did not significantly affect the viability of fused cells. The fusion products were not bound to the surface of the fusion vessel, so they could be isolated with a micropipette immediately after fusion.

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Abbreviations

PEG:

polyethylene glycol

DMSO:

dimethylsulfoxide

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Authors and Affiliations

  1. Advanced Genetic Sciences, Inc., P.O. Box 1373, 66502, Manhattan, KS, USA

    Laszlo Menczel & Kathy Wolfe

Authors
  1. Laszlo Menczel
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  2. Kathy Wolfe
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Communicated by J. Widholm

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Menczel, L., Wolfe, K. High frequency of fusion induced in freely suspended protoplast mixtures by polyethylene glycol and dimethylsulfoxide at high pH. Plant Cell Reports 3, 196–198 (1984). https://doi.org/10.1007/BF00270199

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  • DOI: https://doi.org/10.1007/BF00270199

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