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Clonal propagation of Stevia rebaudiana Bertoni by stem-tip culture

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Abstract

Clonal propagation of Stevia rebaudiana has been established by culturing stem-tips with a few leaf primordia on an agar medium supplemented with a high concentration (10 mg/l) of kinetin. Anatomical examination has suggested that these multiple shoots originate from a number of adventitious buds formed on the margin of the leaf. Innumerable shoots can be obtained by repeating the cycle of multiple-shoot formation from a single stem-tip of Stevia. These shoots produce roots when transferred to a medium containing NAA (0.1 mg/l) without kinetin. The regenerated plantlets can be transplanted to soil.

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Authors and Affiliations

  1. Research Laboratory, Maruzen Kasei Co., Ltd., Mukaihigashi, 722, Onomichi, Japan

    Yukiyoshi Tamura & Shigeharu Nakamura

  2. Faculty of Pharmaceutical Sciences, Kyoto University, 606, Kyoto, Japan

    Hiroshi Fukui & Mamoru Tabata

Authors
  1. Yukiyoshi Tamura
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  2. Shigeharu Nakamura
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  3. Hiroshi Fukui
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  4. Mamoru Tabata
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Communicated by J. Widholm

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Tamura, Y., Nakamura, S., Fukui, H. et al. Clonal propagation of Stevia rebaudiana Bertoni by stem-tip culture. Plant Cell Reports 3, 183–185 (1984). https://doi.org/10.1007/BF00270195

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  • DOI: https://doi.org/10.1007/BF00270195

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