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Conditions for isolation and regeneration of viable protoplasts of oil palm (Elaeis guineensis)

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Abstract

Protoplasts were isolated from cell cultures of oil palm (Elaeis Guineensis). The protoplasts were cultured on a “nurse” medium containing oil palm cells in the presence of which protoplasts formed cell walls and divided to form cell cultures.

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Abbreviations

NAA:

α-naphthalene acetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

FDA:

fluorescein diacetate

TEM:

thin-section electron microscopy

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Communicated by J. Potrykus

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Bass, A., Hughes, W. Conditions for isolation and regeneration of viable protoplasts of oil palm (Elaeis guineensis) . Plant Cell Reports 3, 169–171 (1984). https://doi.org/10.1007/BF00270191

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  • DOI: https://doi.org/10.1007/BF00270191

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