Abstract
Oleasters are olive genotypes that range from wild to feral. They are tolerant to biotic and abiotic stresses and are easily propagated. This study aims to increase the variability of olive trees by somatic hybridization of protoplasts from cultivated olive trees with those from oleasters. Firstly, protoplast isolation of Algerian olive varieties ‘Chemlal’ and ‘Azaradj’ and oleaster rootstock was studied. Of all the cell wall–digesting enzymes tested, the combination of 1.5% driselase and 0.05% pectolyase was optimal for protoplast yield, regardless of variety. In general, callus explants gave the best protoplast yields compared to mesophyll tissue. Chemical fusion was performed with polyethylene glycol (PEG 1540). The obtained protoplasts were cultured in different systems with modified Murashige and Skoog medium supplemented with 90 g L−1 mannitol and 10 g L−1 sucrose, auxins, and cytokinins. Cell divisions and microcolonies of microcalluses took place in liquid culture, solid and liquid culture, and in a culture system that was composed of filter paper discs immersed in liquid medium but not in agarose beads in which the protoplasts were embedded and surrounded by liquid culture.
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This research was financed by Ministry of Higher Education and Scientific Research of Algeria. We dedicate this work in memory of our ex-supervisor Professor KAID HARCHE Meriem.
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Sahouli, S., Abdeddaim, K.K. & Werbrouck, S.P.O. Isolation, chemical fusion, and culture systems for olive (Olea europaea L.) protoplasts. In Vitro Cell.Dev.Biol.-Plant 58, 664–670 (2022). https://doi.org/10.1007/s11627-022-10274-9
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DOI: https://doi.org/10.1007/s11627-022-10274-9