Skip to main content
SpringerLink
Log in

Isolation and growth of protoplasts from cell suspensions of Pinus contorta dougl. ex loud

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Cell suspensions were initiated from embryo derived calli of Pinus contorta. Some of these cell lines could be maintained in culture for at least one year without reduced growth.

A high yield of protoplasts was obtained from the cell suspensions. The protoplasts started to divide after two days and cell clusters could be observed after about two weeks. The growth phase of the cell suspensions was very important for the division of protoplasts. Only protoplasts isolated from suspensions in an actively dividing phase were able to divide with a high frequency and to give rise to cell clusters.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

1-naphthaleneacetic acid

References

  • Arnold S von, Eriksson T (1981) Can. J. Bot. 59: 870–874.

    Google Scholar 

  • David A, David H (1979) Z. Pflanzenphysiol. 94: 173–177.

    Google Scholar 

  • David H, David A, Mateille T (1982) Physiol. Plant. 56: 108–113.

    Google Scholar 

  • Eriksson T (1966) Physiol. Plant. 19: 900–910.

    Google Scholar 

  • Kao K.N., Constabel F., Michayluk M.R., Gamborg O.L. (1974) Planta 120: 215–227.

    Google Scholar 

  • Kirby E.G. (1980) In: Sala F., Parisi B., Cella R., Ciferri O. (eds.) Plant Cell Cultures: Results and Perspectives, Developments in Plant Biology 5, Elsevier/North-Holland, pp. 289–293.

    Google Scholar 

  • Kirby E.G., Cheng T.Y. (1979) Plant Sci. Lett. 14: 145–154.

    Google Scholar 

  • Nagata T., Takebe I. (1970) Planta 92: 301–308.

    Google Scholar 

  • Nagy J.I., Maliga P. (1976) Z. Pflanzenphysiol. 78: 453–455.

    Google Scholar 

  • Strauss A., Potrykus I. (1980) Physiol. Plant. 48: 15–20.

    Google Scholar 

  • Street H.E. (1973) In: Street H.E. (ed.) Plant Tissue and Cell Culture Botanical Monographs vol. 11. Blackwells, London. pp. 59–99.

    Google Scholar 

  • Vasil I.K., Vasil V. (1980) In: Vasil I.K. (ed.) International Review of Cytology, Suppl. 11 B, Academic Press. pp. 1–19.

  • Webb K.J. (1981) In: Proceedings of the international workshop “In vitro” cultivation of forest tree species. Fontainbleau 1981. pp. 217–226.

  • Winton L.L., Parham R.A., Kaustinen H.M. (1975). Genetics and Physiology Notes no 20, Inst. of Paper Chem. Appleton, Wisconsin.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Physiological Botany, University of Uppsala, Box 540, S-75121, Uppsala, Sweden

    I. C. Hakman & S. von Arnold

Authors
  1. I. C. Hakman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. von Arnold
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hakman, I.C., von Arnold, S. Isolation and growth of protoplasts from cell suspensions of Pinus contorta dougl. ex loud. Plant Cell Reports 2, 92–94 (1983). https://doi.org/10.1007/BF00270174

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00270174

Keywords

Search

Navigation