Abstract
A system of liquid batch culture of tobacco (Nicotiana tabacum cv NC2512) is described in which up to 2% nicotine accumulates on a dry weight basis. Nicotine accumulation is first detected in cultures when medium phosphate is completely depleted. By reducing initial medium phosphate to 1/10th levels normally employed, alkaloid accumulation is accelerated while the raising of initial medium sucrose (30–50 g 1−1) results in a five-fold increase in peak accumulation of nicotine. The roles of medium phosphate and sucrose levels in culture growth and nicotine production are discussed.
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Mantell, S.H., Pearson, D.W., Hazell, L.P. et al. The effect of initial phosphate and sucrose levels on nicotine accumulation in batch suspension cultures of Nicotiana tabacum L.. Plant Cell Reports 2, 73–77 (1983). https://doi.org/10.1007/BF00270169
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DOI: https://doi.org/10.1007/BF00270169