Skip to main content
SpringerLink
Log in

Continuous culture growth of photoautotrophic cell suspensions from Chenopodium rubrum

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

The construction and operation of a continuous culture system for the propagation of cell suspensions from Chenopodium rubrum under photoautotrophic conditions has been described. A dilution rate of 0.16/day gave an equilibrium culture density of 1,100,000 cells/ml and a mean doubling time of 150 hours. During continuous culture steady state conditions with respect to nutrient uptake, cell protein and chlorophyll content, starch accumulation, in vitro activities of enzymes related to different metabolic pathways could be established. Photosynthetic CO2 assimilation of steady state cells was about 100 mol CO2/mg chlorophyll x hour. Dark CO2 fixation was 3% of the light values.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

C. rubrum:

Chenopodium rubrum

DTE:

dithioerythritol

EDTA:

ethylene-diamine-tetraacetic acid

HEPES:

N-2-hydroxy-ethylpiperazine-N-2-ethansulfonic acid

S.E.x:

standard error of the mean

References

  • Bergmeyer HU (1970) Methoden der enzymatischen Analyse, Verlag Chemie Weinheim

  • Dalton CC (1980) J. exp. Bot. 31: 791–804

    Google Scholar 

  • Fowler MW (1977) In: Barz W, Reinhard E, Zenk MH (eds.) Plant Tissue Culture and Its Biotechnological Application, Springer Verlag Berlin, Heidelberg, New York

    Google Scholar 

  • Hüsemann W, Plohr A, Barz W (1979) Protoplasma 100: 101–112

    Google Scholar 

  • Hüsemann W (1981) Protoplasma 109: 415–431

    Google Scholar 

  • Hüsemann W (1982) Protoplasma 113: 214–220

    Google Scholar 

  • Jordan DB, Fletcher JS (1979) Plant Science Letters 17: 95–99

    Google Scholar 

  • Laetsch WM, Stetler DA (1965) Am. J. Bot. 52: 798–804

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–479

    Google Scholar 

  • Peel E (1982) Plant Science Letters 24: 147–155

    Google Scholar 

  • Warburg O, Geissler AW, Lorenz S (1961) In: Warburg O (ed.) Weiterentwicklung der zellphysiologischen Methoden, Thieme Verlag Stuttgart

  • Wilson G (1980) In: Fiechter A (ed.) Advances in Biochemical Engineering, Vol. 16, Plant Cell Cultures I, Springer Verlag Berlin, Heidelberg, New York, pp 1–25

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Lehrstuhl für Biochemie der Pflanzen, Universität Münster, Hindenburgplatz 55, D-4400, Münster, FRG

    W. Hüsemann

Authors
  1. W. Hüsemann
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hüsemann, W. Continuous culture growth of photoautotrophic cell suspensions from Chenopodium rubrum . Plant Cell Reports 2, 59–62 (1983). https://doi.org/10.1007/BF00270165

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00270165

Keywords

Search

Navigation