Skip to main content
Log in

Nicotiana chloroplast genome

II. Chloroplast DNA alteration

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The analysis of restriction patterns of chloroplast DNA (ct-DNA) generated by several enzymes reveals that there is a high frequency of ct-DNA alteration in the male sterile but not in the fertile cytoplasma of N. tabacum. The altered restriction patterns and maps suggest that recombination of ct-DNA may have occurred. However, other alternatives such as deletions, insertions, and/or point mutations cannot be ruled out.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Atchison BA, Whitfeld PR, Bottomley W (1976) Comparison of chloroplast DNAs by specific fragmentation with EcoRI endonuclease. Mol Gen Genet 148:263–269

    Google Scholar 

  • Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplasmic fusion. Nature 281:401–403

    Google Scholar 

  • Berbec' J, Berbec' A (1976) Growth and development of Nicotiana tabacum L. from reconstitution in the cytoplasm of Nicotiana glauca Grah Genet Pol 17:309–318

    Google Scholar 

  • Berbec' JA (1974) Cytoplasmic male sterile mutation from Nicotiana tabacum L. F. Pflanzenzuechtung 73:204–216

    Google Scholar 

  • Eberhard WG (1980) Evolutionary consequences of intercellular organelle competition. Q Rev Biol 55:231–249

    Google Scholar 

  • Frankel R, Scowcroft NR, Whitfeld PR (1979) Chloroplast DNA variation in isonuclear male-sterile lines of Nicotiana. Mol Gen Genet 169:129–135

    Google Scholar 

  • Goodspeed TH (1954) The Genus-Nicotiana. Chron Bot, Waltham, Mass., 636 pp

    Google Scholar 

  • Gray JC, Kung SD, Wildman SG, Sheen SJ (1974) Origin of N. tabacum L. detected by polypeptide composition of fraction 1 protein. Nature 252:226–227

    Google Scholar 

  • Helling RB, Goodman HW, Boyer HW (1974) An analysis of endonuclease R EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol 14:1235–1241

    Google Scholar 

  • Jurgenson JE (1980) Nicotiana tabacum chloroplast DNA: structure and gene content, Ph D. Dissertation University of Arizona, 149 pp

  • Jurgenson JE, Bourque DP (1980) Mapping of RNA genes in an inverted region in N. tabacum chloroplast DNA. Nucl Acids Res 8:3505–3516

    Google Scholar 

  • Kirk JTO (1971) Chloroplast structure and biosynthesis. Annu Rev Biochem 40:161–196

    Google Scholar 

  • Kolodner R, Tewari KK (1975) The molecular size and conformation of chloroplast DNA from higher plants. Biochim Biophys Acta 402:372–390

    Google Scholar 

  • Kolodner R, Tewari KK (1979) Inverted repeats in chloroplast DNA from higher plants. Proc Natl Acad Sci USA 76:41–45

    Google Scholar 

  • Kung SD (1976) Tobacco fraction 1 protein: a unique genetic marker. Science 191:429–434

    Google Scholar 

  • Kung SD (1977) Expression of chloroplast genomes in high plants. Annu Rev Plant Physiol 28:401–437

    Google Scholar 

  • Kung SD, Williams JP (1969) DNA from broad bean. Biochim Biophys Acta 195:434–445

    Google Scholar 

  • Kung SD, Zhu SY, Shen GF, Chen K (1981) Nicotiana chloroplast genome III. Chloroplast DNA evolution. Theor Appl Genet (in press)

  • Pittarelli GW, Stavely JR (1975) Direct hybridization of Nictotiana repanda X N. tabacum. J Herd 66:281–284

    Google Scholar 

  • Rhodes PR, Kung SD (1981) Chloroplast DNA isolation: Purity achieved without nuclease digestion. Can J Biochem (submitted)

  • Rhodes PR, Zhu YS, Kung SD (1981) Nicotiana chloroplast genome I. Chloroplast DNA diversity. Mol Gen Genet 182:106–111

    Google Scholar 

  • Vaughn KC, DeBonte LR, Wilson KG, Schaeffer GW (1980) Organelle alteration as a mechanism for maternal inheritance. Science 208:196–198

    Google Scholar 

  • Vedel F, Quetier F, Bayen M (1976) Specific cleavage of chloroplast DNA from higher plants by EcoRI restriction nuclease. Nature 263:440–442

    Google Scholar 

  • Walbot V (1977) Use of silica sol step gradients to prepare bundle sheath and mesophyll chloroplasts from Panicum maximum. Plant Physiol 60:102–108

    Google Scholar 

  • Wildman SG, Lu-Liao C, Wong-Staal F (1973) Maternal inheritance, cytology, and macromolecular composition of defective chloroplasts in variegated mutant of Nicotiana tabacum. Planta 113:293–312

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by G. Melchers

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kung, S.D., Zhu, Y.S., Chen, K. et al. Nicotiana chloroplast genome. Molec. Gen. Genet. 183, 20–24 (1981). https://doi.org/10.1007/BF00270132

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00270132

Keywords

Navigation