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Somatic embryogenesis in cell cultures of Glycine species

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Abstract

This report describes the development of procedures for the production of somatic embryos in cell cultures of Glycine species including soybean. The conditions for callus induction and initiation of rapidly growing cell suspension cultures were defined. Methods for inducing embryogenesis were tested on 16 lines of several Glycine species and cultivars of soybean. The SB-26 Culture of a G. soja gave the best results and was used in the experiments. Embryogenesis required the presence of picloram or 2,4-D. AMO 1618, CCC, PP-333 and Ancymidol enhanced the embryogenesis frequency. Plants of the G. soja (SB-26) were grown to maturity from seed-derived shoot tips. Characteristics of the plants are discussed.

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Abbreviations

BA:

Benzyladenine

NAA:

Naphthaleneacetic acid

IAA:

Indoleacetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

GA:

Gibberellic acid

AMO:

1618, CCC, PP-333, MS, B5 (See text)

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Authors and Affiliations

  1. International Plant Research Institute, 94070, San Carlos, CA, USA

    O. L. Gamborg, B. P. Davis & R. W. Stahlhut

  2. Health Science Center, University of Texas, Dallas, TX, USA

    B. P. Davis

  3. Department of Agronomy, University of Illinois, Urbana, IL, USA

    R. W. Stahlhut

  4. Genentech, Inc., 460 Point San Bruno Blvd., 94080, South San Francisco, CA, USA

    O. L. Gamborg

Authors
  1. O. L. Gamborg
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  2. B. P. Davis
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  3. R. W. Stahlhut
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Gamborg, O.L., Davis, B.P. & Stahlhut, R.W. Somatic embryogenesis in cell cultures of Glycine species. Plant Cell Reports 2, 209–212 (1983). https://doi.org/10.1007/BF00270106

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  • DOI: https://doi.org/10.1007/BF00270106

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