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Tissue and protoplast culture studies in Lycopersicon esculentum miller var. flammatum lehm. cv. ‚Bonner Beste‘ and its mutant chloronerva

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Abstract

Methods of shoot and plant development from cotyledon explants of the tomato cultivar ‘Bonner Beste’ and its mutant chloronerva are described. Cotyledon mesophyll protoplasts of the mutant chloronerva have been isolated and cultured, and induced to regenerate shoots and plants. By grafting regenerated shoots of the chloronerva mutant on rootstocks of the cultivar ‘Bonner Beste’ plants were obtained producing fruits and seeds. Plants derived from these seeds show all the typical characteristics of the chloronerva mutant.

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Abbreviations

IAA:

indoleacetic acid

IBA:

indolebutyric acid

BA:

6-benzyladenine

MS:

Murashige and Skoog (1962) medium

B5:

Gamborg et al. (1968) medium

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Authors and Affiliations

  1. Akademie der Wissenschaften der DDR, Zentralinstitut für Genetik und Kulturpflanzenforschung Gatersleben, DDR-4325, Gatersleben, Germany

    H. Koblitz & D. Koblitz

Authors
  1. H. Koblitz
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  2. D. Koblitz
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Communicated by J. Potrykus

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Koblitz, H., Koblitz, D. Tissue and protoplast culture studies in Lycopersicon esculentum miller var. flammatum lehm. cv. ‚Bonner Beste‘ and its mutant chloronerva. Plant Cell Reports 2, 194–197 (1983). https://doi.org/10.1007/BF00270102

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  • DOI: https://doi.org/10.1007/BF00270102

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