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Plant regeneration in vitro from leaf tissues derived from cultured immature embryos of Zea mays L.

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Abstract

Maize (Zea mays L.) A188 calluses derived from leaf tissues of in vitro grown seedlings were initiated and maintained on Murashige and Skoog medium supplemented with 2 mg/1 2,4-dichlorophenoxyacetic acid (2,4-D). The calluses produced green leafy structures and subsequently plantlets upon transfer to N6 medium containing 2 mg/1 2,4-D and 0.1 mg/1 zeatin. An embryo-like structure with a green prominent coleoptile and a scutellum-like body was also obtained.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

2-MS:

2 mg/1 2,4-D in MS medium

N6:

(Chu et al. 1975)

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Authors and Affiliations

  1. International Plant Research Institute, 830 Bransten Road, 94070, San Carlos, CA, USA

    Y. -F. Chang

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  1. Y. -F. Chang
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Communicated by O. L. Gamborg

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Chang, Y.F. Plant regeneration in vitro from leaf tissues derived from cultured immature embryos of Zea mays L.. Plant Cell Reports 2, 183–185 (1983). https://doi.org/10.1007/BF00270098

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  • DOI: https://doi.org/10.1007/BF00270098

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