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Pollen embryogenesis in anther cultures of Solanum carolinense L.

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Abstract

The direct differentiation of bicellular pollen grains of Solanum carolinense L. (Horse-nettle; Solanaceae) into embryoids and plantlets was induced by culturing whole anthers on Murashige and Skoog's medium supplemented with IAA. The highest frequency of embryogenic induction occurred at 10 mg/l IAA. Developmentally, both the generative and vegetative cells of the pollen grain contributed to embryoid formation whose pattern of development was similar to that of zygotic embryos. In a previous study, it was show that 2,4-D promoted callus formation by pollen grains in cultured anthers of S. carolinense. It appears then that there are two distinct pathways of androgenesis in this species that are determined by the type of auxin present in the medium.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

IAA:

indole-3-acetic acid

BA:

benzyladenine

KIN:

kinetin

MS:

Murashige and Skoog

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Authors and Affiliations

  1. Department of Biology, The University of North Carolina, 28223, Charlotte, NC, USA

    Thomas L. Reynolds

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  1. Thomas L. Reynolds
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Communicated by F. Constabel

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Reynolds, T.L. Pollen embryogenesis in anther cultures of Solanum carolinense L.. Plant Cell Reports 5, 273–275 (1986). https://doi.org/10.1007/BF00269820

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  • DOI: https://doi.org/10.1007/BF00269820

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