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Isolation, culture and callus regeneration of protoplasts of wild and cultivated Helianthus species

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Abstract

Protoplasts were isolated from seedling roots, hypocotyls, and cotyledons of four cultivars of Helianthus annuus and from leaves of axenic shoot cultures of the wild species H. praecox, H. scaberimus and H. rigidus. Optimal culture conditions were established for the respective protoplast systems, using the agarose bead method of culture. Protoplast division was induced for all the species examined. In the case of the cultivars of H. annuus, hypocotyl and cotyledon protoplast division was sustained leading to callus formation, which in turn, could be induced to produce roots and organised meristematic regions in the presence of NAA and 6-BAP.

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Abbreviations

6-BAP:

6-benzylaminopurine

NAA:

α-naphthalene acetic acid

IAA:

indole-3-acetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

MS:

Murashige and Skoog

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Authors and Affiliations

  1. Institute of Genetics, Bulgarian Academy of Sciences, 113, Sofia, Bulgaria

    N. E. Bohorova

  2. Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, Nottingham, UK

    E. C. Cocking & J. B. Power

Authors
  1. N. E. Bohorova
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  2. E. C. Cocking
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  3. J. B. Power
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Communicated by I. Potrykus

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Bohorova, N.E., Cocking, E.C. & Power, J.B. Isolation, culture and callus regeneration of protoplasts of wild and cultivated Helianthus species. Plant Cell Reports 5, 256–258 (1986). https://doi.org/10.1007/BF00269815

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  • DOI: https://doi.org/10.1007/BF00269815

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