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Genetic studies of the ribosomal proteins in Escherichia coli

VI. Determination of chromosomal loci for several ribosomal protein components using a hybrid strain between Escherichia coli and Salmonella typhimurium

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Summary

Salmonella typhimurium L+15-11 was shown to have four 30s and six 50s ribosomal protein components which were chromatographically distinguishable from their counterparts of Escherichia coli K-12 JC411E01. A hybrid strain between E. coli K-12 JC411E01 and S. typhimurium L+15-11, having ribosomes of S. typhimurium-type protein composition and being sensitive to phage Plkc was isolated by conjugation between these two species.

Transduction experiments by phage Plkc between the above hybrid strain and E. coli K-12 JC411ES01 showed that characters of all the S. typhimurium-type ribosomal protein components could be cotransduced with the streptomycin or spectinomycin resistance marker, indicating that the genes specifying these ten ribosomal protein components were located in the str region on the chromosome.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Kasumi-cho, Hiroshima, Japan

    Satoshi Dekio, Renkichi Takata & Syozo Osawa

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  1. Satoshi Dekio
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  2. Renkichi Takata
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  3. Syozo Osawa
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Communicated by H. G. Wittmann

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Dekio, S., Takata, R. & Osawa, S. Genetic studies of the ribosomal proteins in Escherichia coli . Molec. Gen. Genet. 109, 131–141 (1970). https://doi.org/10.1007/BF00269649

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  • DOI: https://doi.org/10.1007/BF00269649

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