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Somatic embryogenesis from leaf and petiole callus cultures of Gossypium hirsutum L.

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Abstract

Leaf discs from four strains and petioles from six strains of Gossypium hirsutum were cultured on a variety of media. Callus formed from explants on all media, though embryogenesis was highly specific. Embryos formed from only three strain x media combinations. A small percentage of these embryos developed into plantlets. These results demonstrate that cotton plants can be obtained from leaf tissue explants.

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Abbreviations

BA:

benzyladenine

2,4-D:

2,4-dichlorophenoxyacetic acid

GA3 :

gibberellic acid

Kn:

Kinetin

NAA:

1-naphthalene acetic acid

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Authors and Affiliations

  1. Department of Plant and Soil Science, Texas Tech University, 79409, Lubbock, TX, USA

    Nick J. Gawel, Arelli P. Rao & Carol D. Robacker

Authors
  1. Nick J. Gawel
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  2. Arelli P. Rao
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  3. Carol D. Robacker
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Additional information

Communicated by G. C. Phillips

College of Agricultural Sciences Publication Number T-4-193; this research was partially funded by the USDA-ARS Plant Stress and Water Conservation Research Program

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Gawel, N.J., Rao, A.P. & Robacker, C.D. Somatic embryogenesis from leaf and petiole callus cultures of Gossypium hirsutum L.. Plant Cell Reports 5, 457–459 (1986). https://doi.org/10.1007/BF00269641

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  • DOI: https://doi.org/10.1007/BF00269641

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