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Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase

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Abstract

Genetic transformation of flax (Linum usitatissimum) has been achieved using an A. tumefaciens strain carrying a non-oncogenic Ti plasmid-derived vector containing a chimaeric npt-II gene and a wild type nopaline synthase gene. Fertile, transformed shoots were most easily obtained from Kmr callus developing on hypocotyl sections. The totipotency of the Kmr callus was dependent upon its origin. T-DNA was visualised by Southern blotting in all Kmr tissues. Efficient expression of nopaline synthase and the chimaeric npt-II gene was found in transformed Kmr callus and regenerated shoots.

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Abbreviations

npt-II :

neomycin phosphotransferase II gene

NPT-II:

neomycin phosphotransferase II

nos:

nopaline synthase gene promoter

Kmr :

kanamycin resistant

BAP:

6-benzylaminopurine

NAA:

α-naphthaleneacetic acid

MSD4×2:

medium D4×2 based on Murashige & Skoog medium (see Scott & Draper, 1987)

References

  • Barakat M.N., Cocking E.C. (1983) Plant Cell Reports 2: 314–317.

    Google Scholar 

  • Bennet M.D., Smith J.B. (1976) Phil. Trans. Roy. Soc. Lond. B. 274: 227–273.

    Google Scholar 

  • Gamborg O.L., Shyluk J.P. (1976) Bot. Gaz. 137: 301–306.

    Google Scholar 

  • Gamborg O.L., Miller R.A., Ojima K. (1968) Exp. Cell Res. 50: 151–158.

    Google Scholar 

  • Hain, R., Stabel, P., Czernilofsky, A.P., Steinbiss, H.H., Herrera-Estrella, L. Schell, J. (1985) Mol. Gen. Genet. 199: 161–168.

    Google Scholar 

  • Hepburn A.G., Clarke L.E., Blundy K.S., White J. (1983) J. Mol. Appl. Genet. 2: 211–224.

    Google Scholar 

  • Johnson C.M., Stout R.R., Broyer T.C., Carlton A.B. (1957) Plant and Soil 8: 337–353.

    Google Scholar 

  • Link G.K., Eggers V. (1946) Bot. Gaz. 107: 441–454.

    Google Scholar 

  • McHughen A., Swartz M. (1984) J. Plant Physiol. 117: 109–117.

    Google Scholar 

  • Murray B.E., Handyside R.J., Keller W.A. (1977) Can. J. Genet. 19: 177–186.

    Google Scholar 

  • Rybczynski J.J. (1975) Genet. Pol. 16: 161–172.

    Google Scholar 

  • Scott R.J., Draper J. (1987) Plant Mol. Biol. 8: 265–274.

    Google Scholar 

  • Zambryski P., Joos H., Genetello C., Leemans J., Van Montagu M., Schell J. (1983) EMBO J. 2: 2143–2150.

    Google Scholar 

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Authors and Affiliations

  1. Department of Botany, University of Leicester, University Road, LE1 7RH, Leicester, UK

    Nazir Basiran, Philip Armitage, Roderick John Scott & John Draper

Authors
  1. Nazir Basiran
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  2. Philip Armitage
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  3. Roderick John Scott
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  4. John Draper
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Communicated by I. Potrykus

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Basiran, N., Armitage, P., Scott, R.J. et al. Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase. Plant Cell Reports 6, 396–399 (1987). https://doi.org/10.1007/BF00269571

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  • DOI: https://doi.org/10.1007/BF00269571

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