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Studies on proteins of animal ribosomes

XV. Proteins of the small subunit of rat liver ribosomes: Isolation, amino acid composition, tryptic peptides and molecular weights

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Summary

  1. 1)

    24 proteins of the small subunit of rat liver ribosomes were isolated by detachment of ribosomal proteins from the subunits bound to DEAE-Sephadex A-25 by LiCl and β-chlorethanol-HCl and subsequent ion exchange chromatography and gel filtration of the prefractionated protein mixtures.

  2. 2)

    The amino acid compositions of the isolated proteins are fairly similar, but most of them are different from each other.

  3. 3)

    The tryptic peptide patterns of the isolated proteins point to differences in the primary structure.

  4. 4)

    The molecular weights estimated by different methods are in the range from 9700 to 30 600. The mean value of the molecular weights is 19 200.

  5. 5)

    The number of proteins and the sum of their molecular weights point to an average of one copy of each protein per small ribosomal subunit.

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Authors and Affiliations

  1. Department of Cell Physiology, Central Institute of Molecular Biology of the Academy of Sciences of GDR, 1115, Berlin

    P. Westermann & H. Bielka

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  1. P. Westermann
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  2. H. Bielka
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Communicated by H. G. Wittmann

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Westermann, P., Bielka, H. Studies on proteins of animal ribosomes. Molec. Gen. Genet. 126, 349–356 (1973). https://doi.org/10.1007/BF00269444

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  • DOI: https://doi.org/10.1007/BF00269444

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