Summary
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1)
From the total protein mixture of rat liver ribosomes 31 proteins were isolated by CM-cellulose chromatography and subsequent gel filtration on Sephadex G 100 or Biogel P 10.
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2)
Molecular weights of the proteins estimated by a sedimentation equilibrium method range from 10000 to 30000. The values are in good agreement with data obtained from gel filtrations on Biogel P 10 in 1.0 M propionic acid.
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3)
The amino acid compositions of the proteins are similar. They differ only in the content of certain amino acids.
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4)
Circular dichroic spectra of the proteins between 200 and 300 nm indicate a small content of ordered structures.
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Communicated by H. G. Wittmann
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Westermann, P., Bielka, H. & Böttger, M. Studies on proteins of animal ribosomes. Molec. Gen. Genet. 111, 224–234 (1971). https://doi.org/10.1007/BF00433107
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DOI: https://doi.org/10.1007/BF00433107