Skip to main content
SpringerLink
Log in

Morphogenesis and plant regeneration from cultured endosperm of Emblica officinalis Gaertn

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Mature endosperm of Emblica Officinalis (Euphorbiaceae) formed a continously growing callus on MS medium supplemented with an auxin (2,4-D or IAA) and a cytokinin (K or BAP). Subculture of callus on MS with BAP (0.2 mg/l) and IAA (0.1 mg/l) resulted in formation of shoots and embryo-like structures in 50 and 8 per cent cultures, respectively. Regeneration of shoots was more frequent when both BAP (0.2 mg/l) and IAA (0.1 mg/l) were present than on BAP (0.2 mg/l) alone. The embryo-like structures produced plantlets.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BAP:

benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

GA3 :

gibberellic acid

IAA:

indoleacetic acid

K:

kinetin

NAA:

naphthaleneacetic acid

PDB:

para-dichlorobenzene

References

  • Bapat V A, Narayanaswami S (1977) Indian J. Exp. Biol. 15: 294–296

    Google Scholar 

  • Bhojwani S S (1966) Phytomorphology 16: 349–353.

    Google Scholar 

  • Bhojwani S S (1971) New Phytol. 70:761–766.

    Google Scholar 

  • Bhojwani S S (1984) In: I K Vasil (ed) Cell Culture and Somatic Cell Genetics of Plants, Vol 1, Academic Press, New York, pp 258–268.

    Google Scholar 

  • Gui Y, Mu X, Xu T (1982) Acta Bot. Sin. 24: 216–221

    Google Scholar 

  • Lakshmi Sita G, Raghava Ram N V, Vaidyanathan C S (1980) Pl. Sci. Letts. 20:63–69.

    Google Scholar 

  • Mu S-K, Liu S-Q, Zhou Y-K, Qian N F, Zhang P, Zie H-X, Zhang F S, Yan Z L (1977) Scientia Sinica 20: 370–376.

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497

    Google Scholar 

  • Sastri B N (ed) (1952) The Wealth of India, Vol III, Council of Scientific & Industrial Research, New Delhi, pp 168.

    Google Scholar 

  • Satsangi A, Mohan Ram H Y (1965) Phytomorohology 15: 20–30.

    Google Scholar 

  • Shu-quiong L, Jia-qu L (1980) Acta Bot. Sin. 22: 198–199

    Google Scholar 

  • Sehgal C B (1969) Beitr. Biol. Pflanz. 46:233–238.

    Google Scholar 

  • Sehgal C B (1974) Curr. Sci. 43: 38–40.

    Google Scholar 

  • Sehgal C B, Narang KJ, Arora S (1981) Beitr. Biol. Pflanzen 55: 385–392.

    Google Scholar 

  • Sethi M, Rangaswamy N S (1976) Curr. Sci. 45: 109–111.

    Google Scholar 

  • Srivastava P S (1971) Studies on mature endosperm of Jatropha, Putranjiva and Ricinus Ph D Thesis, Univ. Delhi, Delhi, India.

    Google Scholar 

  • Wang T-Y, Chang C-J (1978) Scientia Sinica 21: 823–827.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, University of Delhi, 110 007, Delhi, India

    C. B. Sehgal & Sunilla Khurana

Authors
  1. C. B. Sehgal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sunilla Khurana
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by I. K. Vasil

(née Arora)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sehgal, C.B., Khurana, S. Morphogenesis and plant regeneration from cultured endosperm of Emblica officinalis Gaertn. Plant Cell Reports 4, 263–266 (1985). https://doi.org/10.1007/BF00269373

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00269373

Keywords

Search

Navigation