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Intracellular distribution of phosphate in cultured Humulus lupulus cells growing at elevated exogenous phosphate concentrations

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Abstract

The distribution of inorganic phosphate (Pi) between the cytoplasm and the vacuole of Humulus lupulus L. cells grown in suspension culture at different exogenous Pi levels was examined by 31-P nuclear magnetic resonance. In growing cells excess Pi accumulated in the vacuoles and the inhibitory effect of high exogenous Pi was not associated with a change in the cytoplasmic Pi level or with a change in the cytoplasmic pH.

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Abbreviations

MES:

2-(N-morpholino)ethanesulphonic acid

NMR:

nuclear magnetic resonance

Pi:

inorganic phosphate

ppm:

parts per million

References

  • Gamborg O L (1966) Can. J. Biochem. 44: 791–799

    Google Scholar 

  • Gamborg O L, Miller R A, Ojima K (1968) Exp. Cell Res. 50: 151–158

    Google Scholar 

  • Hughes W A, Bociek S M, Barrett J N, Ratcliffe R G (1983) Bioscience Rep. 3: 1141–1148

    Google Scholar 

  • Kime M J, Loughman B C, Ratcliffe R G, Williams R J P (1982) J. exp. Bot. 33: 656–669

    Google Scholar 

  • Knobloch K-H, Beutnagel G, Berlin J (1981) Planta 153: 582–585

    Google Scholar 

  • Knobloch K-H (1982) Plant Cell Rep. 1: 128–130

    Google Scholar 

  • Lee R B, Ratcliffe R G (1983a) J. exp. Bot. 34: 1213–1221

    Google Scholar 

  • Lee R B, Ratcliffe R G (1983b) J. exp. Bot. 34: 1222–1244

    Google Scholar 

  • Loughman B C, Ratcliffe R G (1984) In: Lauchli A, Tinker P B (eds) Advances in Plant Nutrition, 1, Praeger (in press)

  • Martin J-B, Bligny R, Rebeille F, Douce R, Leguay J-J, Mathieu Y, Guern J. (1982) Plant Physiol. 70: 1156–1161

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plantarum 15: 473–497

    Google Scholar 

  • Rebeille F, Bligny R, Douce R (1982) Arch. Biochem. Biophys. 219: 371–378

    Google Scholar 

  • Rebeille F, Bligny R, Martin J-B, Douce R (1983) Arch. Biochem. Biophys. 225: 143–148

    Google Scholar 

  • Roberts J K M, Ray P M, Wade-Jardetzky N, Jardetzky O (1980) Nature 283: 870–872

    Google Scholar 

  • Roberts J K M, Wade-Jardetzky N, Jardetzky O (1981) Biochemistry 20: 5389–5394

    Google Scholar 

  • Robins R J, Furze J M, Rhodes M J C (1985) Phytochemistry (in press)

  • Schenk R U, Hildebrandt A C (1972) Can. J. Bot. 50: 199–204

    Google Scholar 

  • Thom M, Maretzki A, Komor E, Sakai W S (1981) Plant Cell Tis. Org. Cult. 1: 3–14

    Google Scholar 

  • Wray V, Schiel O, Berlin J (1983) Z. Pflanzenphysiol. 112: 215–220

    Google Scholar 

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Author notes

  1. R. G. Ratcliffe

    Present address: Department of Agricultural Science, Parks Road, OX1 3PF, Oxford, UK

Authors and Affiliations

  1. A.F.R.C. Food Research Institute, Colney Lane, NR4 7UA, Norwich, UK

    R. J. Robins & R. G. Ratcliffe

Authors
  1. R. J. Robins
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  2. R. G. Ratcliffe
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Communicated by M. H. Zenk

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Robins, R.J., Ratcliffe, R.G. Intracellular distribution of phosphate in cultured Humulus lupulus cells growing at elevated exogenous phosphate concentrations. Plant Cell Reports 3, 234–236 (1984). https://doi.org/10.1007/BF00269300

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  • DOI: https://doi.org/10.1007/BF00269300

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