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Somatic embryogenesis and regeneration of plants in the bamboo Dendrocalamus strictus

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Abstract

Somatic embryogenesis leading to plant regeneration has been achieved in the bamboo, Dendrocalamus strictus, by culturing seeds (caryopses) on B5 basal medium supplemented with 2,4-dichlorophenoxyacetic acid. Callus cultures obtained from the embryonal end of the seeds differentiated chlorophyllous embryoids. On transfer to a germination medium (B5 liquid, sucrose, indolebutyric acid, and ∝ -naphthaleneacetic acid) 40% of the embryoids developed into plantlets. Further development of the plantlets occured on B5 liquid medium (half strength) + sucrose (1%) + IBA (5 × 10−7M) + NAA (10−7M).

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

IBA:

Indolebutyric acid

NAA:

∞-naphthaleneacetic acid

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Authors and Affiliations

  1. Department of Botany, University of Delhi, 110 007, Delhi, India

    I. Usha Rao, I. V. Ramanuja Rao & Vibha Narang

Authors
  1. I. Usha Rao
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  2. I. V. Ramanuja Rao
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  3. Vibha Narang
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Communicated by I. K. Vasil

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Rao, I.U., Ramanuja Rao, I.V. & Narang, V. Somatic embryogenesis and regeneration of plants in the bamboo Dendrocalamus strictus . Plant Cell Reports 4, 191–194 (1985). https://doi.org/10.1007/BF00269286

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  • DOI: https://doi.org/10.1007/BF00269286

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