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Influence of growth conditions on the composition of cell wall polysaccharides from cultured tobacco cells

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Abstract

The sugar composition of cell wall polysaccharides of two tobacco varieties obtained from mesophyll, regenerating protoplasts and cells grown under various conditions were compared. Regenerating protoplasts developed an unusual cell wall with a low cellulose and a high non-cellulosic glucan content. In the presence of different phytohormones compact and friable calli were obtained with cell walls containing low and high arabinose/xylose ratios. The cell walls of compact calli were comparable to those of genuine mesophyll cells. The sugar constituents of cell walls obtained from cells grown in liquid media were different from those of solid calli. The cell wall composition of suspension cultured cells was hardly affected by various combinations of phytohormones, but was altered by high osmolarity of the medium.

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Abbreviations

NAA:

1-naphthaleneacetic acid

6-BA:

6-benzyl-aminopurin

IAA:

indole-3-acetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

CWP:

cell wall polysaccharides

ECP:

extra-cellular polysac-charides

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Authors and Affiliations

  1. Department of Chemistry and Pharmacy, University of Regensburg, D-8400, Regensburg, FRG

    W. Blaschek & G. Franz

Authors
  1. W. Blaschek
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  2. G. Franz
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Communicated by M. H. Zenk

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Blaschek, W., Franz, G. Influence of growth conditions on the composition of cell wall polysaccharides from cultured tobacco cells. Plant Cell Reports 2, 257–260 (1983). https://doi.org/10.1007/BF00269155

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  • DOI: https://doi.org/10.1007/BF00269155

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