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Propagation of Casuarina equisetifolia through axillary buds of immature female inflorescences cultured in vitro

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Abstract

The study of the actinorhizal symbiosis in Casuarina equisetifolia requires an homogenous plant material. Consequently, we devised a method of micropropagation based on the use of immature female inflorescences (IFI) as explants. IFI excised from an adult tree formed multiple buds after 4-week incubation on Murashige and Skoog medium with 0.05 μmol 1−1 NAA and 11.1 μmol 1−1 BAP. The axillary buds evolved into 5–6 cm long shoots 5 weeks after the transfer of IFI on a similar medium except for the addition of activated charcoal. Rooting of the shoots was obtained on a third medium, without BAP or charcoal, but with 1 μmol 1−1 NAA. The plantlets were transferred into soil. Their growth was satisfactory and no plagiotropic tendency was observed.

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Abbreviations

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

IAA:

3-indoleacetic acid

IFI:

immature female inflorescences

NAA:

α-naphtaleneacetic acid

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Authors and Affiliations

  1. Départment de Biologie Végétale, Faculté des Sciences, Dakar, Sénégal

    E. Duhoux

  2. Laboratoire de Microbiologie des sols, ORSTOM, Dakar, Sénégal

    B. Sougoufara

  3. BSSFT (CTFT/ORSTOM/CNRS), 45 bis Avenue de la Belle Gabrielle, F-94736, Nogent-sur-Marne Cedex, France

    Y. Dommergues

Authors
  1. E. Duhoux
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  2. B. Sougoufara
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  3. Y. Dommergues
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Communicated by A. M. Boudet

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Duhoux, E., Sougoufara, B. & Dommergues, Y. Propagation of Casuarina equisetifolia through axillary buds of immature female inflorescences cultured in vitro. Plant Cell Reports 5, 161–164 (1986). https://doi.org/10.1007/BF00269108

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  • DOI: https://doi.org/10.1007/BF00269108

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