Summary
From Escherichia coli, DNA-membrane-complexes have been isolated which contain about 40% of the ribosomes, about 95% of the DNA and nearly all of the nascent RNA. The kinetic data on pulse labeled RNA show an average time of turnover of about 60 sec both for nascent messenger- and nascent ribosomal RNA. A proportion of the polysomes with nascent messenger RNA as well as most of the nascent ribosomal RNA is found in association with membranes, as has been shown by subfractionations of the DNA-membrane-complex involving treatment with DNase and desoxycholate. In this early transient stage, ribosomal precursor RNA already acquires some ribosomal proteins, as has been shown by arginine pulse label. Data on partial release of DNA from the DNA-membrane-complex by treatment with extremely low doses of DNase indicate that messenger RNA synthesis occurs in clusters on the DNA.
The results support models in which, at any given time, RNA synthesis proceeds mainly in sections of the DNA close to the membrane. Thereby the DNA is linked to the membrane via nascent RNA contained in ribosomal precursors as well as via nascent messenger RNA on membrane-bound polysomes.
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Adesnik, M., Levinthal, C.: Synthesis and maturation of ribosomal RNA in Escherichia coli. J. molec. Biol. 46, 281 (1969)
Baker, R., Yanowsky, Ch.: Transcription initiation frequency for the tryptophan operon of Escherichia coli. Cold Spr. Harb. Symp. quant. Biol. 35, 467 (1970)
Flessel, R. P.: Ph. D. thesis, Massachusetts Institute of Technology, 1969
Gierer, L., Gierer, A.: Synthesis of ribosomal proteins and formation of ribosomes in Escherichia coli. J. molec. Biol. 34, 293 (1968)
Godson, G. N., Sinsheimer, R. L.: Use of Brij lysis as a general method to prepare polyribosomes from Escherichia coli. Biochim. biophys. Acta (Amst.) 149, 489 (1967)
Haywood, A. M.: Cellular site of Escherichia coli ribosomal RNA synthesis. Proc. nat. Acad. Sci. (Wash.) 68, 435 (1971)
Knippenberg, P., van, Duijts, G. A. H.: Polyribosomes of Escherichia coli: II. In vivo and in vitro studies on DNA bound and cytoplasmic polysomes. Molec. gen. Genet. 112, 208 (1971b)
Knippenberg, P., van, Duijts, G. A. H., Euwe, M. S. T.: Polyribosomes of Escherichia coli: I. Isolation of polysomes from a complex of DNA and membrane. Molec. gen. Genet. 112, 197 (1971a)
Mangiarotti, G., Apirion, D., Schlessinger, D., Silengo, L.: Biosynthetic precursors of 30s and 50s ribosomal particles in Escherichia coli. J. Biochem. 7, 456 (1968).
Mangiarotti, G., Schlessinger, D.: Polyribosome metabolism in Escherichia coli: II. Formation and lifetime of messenger RNA molecules, ribosomal subunit couples and polyribosomes. J. molec. Biol. 29, 395 (1967)
Miller, O. L., Beatty, B. R., Hamkalo, B. A., Thomas, C. A.: Electron microscopic visualization of transcription. Cold Spr. Harb. Symp. quant. Biol. 35, 505 (1970)
Morse, D. E., Mosteller, R. D., Yanowsky, Ch.: Dynamics of synthesis translation, and degradation of trp operon messenger RNA in Escherichia coli. Cold Spr. Harb. Symp. quant. Biol. 34, 725 (1969)
Mueller, K., Bremer, H.: Rate of synthesis of messenger ribonucleic acid in Escherichia coli. J. molec. Biol. 38, 329 (1968)
Nierhaus, K., Bordasch, K., Homann, H. E.: Ribosomal proteins: In vivo assembly of Escherichia coli ribosomal proteins. J. molec. Biol. 74, 587 (1973)
Nierlich, D. P.: Regulation of ribonucleic acid synthesis in growing bacterial cells. J. molec. Biol. 72, 751 (1972)
Osawa, S., Otaka, E., Itoh, T., Fukui, T.: Biosynthesis of 50s ribosomal subunit in Escherichia coli. J. molec. Biol. 40, 321 (1969)
Rosenberg, B. H., Cavalieri, L. F.: Shear sensitivity of the Escherichia coli genome: multiple membrane attachment points of the Escherichia coli DNA. Cold Spr. Harb. Symp. quant. Biol. 33, 65 (1868)
Rouvière, J., Lederberg, S., Granboulan, P., Gros, F.: Structural sites of RNA synthesis in Escherichia coli. J. molec. Biol. 46, 413 (1969)
Stonington, O. G., Pettijohn, D. E.: The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex. Proc. nat. Acad. Sci. (Wash.) 68, 6 (1971)
Sypherd, P.: Ribosome development and the methylation of ribosomal ribonucleic acid. J. Bact. 95, 1844 (1968)
Worcel, A., Burgi, E.: On the structure of the folded chromosome of Escherichia coli. J. molec. Biol. 71, 127 (1972)
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Communicated by H. G. Wittmann
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Gierer, L. Nascent ribosomal and messenger RNA in DNA-membrane-complexes of Escherichia coli . Molec. Gen. Genet. 125, 173–187 (1973). https://doi.org/10.1007/BF00268870
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DOI: https://doi.org/10.1007/BF00268870